Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18063 | 5' | -55 | NC_004680.1 | + | 9928 | 0.7 | 0.503505 |
Target: 5'- --cGCGCGGCCAGccaccGCGCCAgcCUUCGa -3' miRNA: 3'- gcuUGCGUCGGUCu----UGCGGUa-GAGGC- -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 4508 | 0.7 | 0.493212 |
Target: 5'- aGGACGCAGCCgccacgguugaGGAgccaACGCaagCUCCGa -3' miRNA: 3'- gCUUGCGUCGG-----------UCU----UGCGguaGAGGC- -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 10624 | 0.75 | 0.269212 |
Target: 5'- aGAcuGCGCAGgaCCuguGGAAUGCCAUCUCCa -3' miRNA: 3'- gCU--UGCGUC--GG---UCUUGCGGUAGAGGc -5' |
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18063 | 5' | -55 | NC_004680.1 | + | 5629 | 1.09 | 0.001277 |
Target: 5'- aCGAACGCAGCCAGAACGCCAUCUCCGc -3' miRNA: 3'- -GCUUGCGUCGGUCUUGCGGUAGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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