Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18248 | 5' | -56.8 | NC_004681.1 | + | 7264 | 0.66 | 0.785266 |
Target: 5'- -gCGGCCGuccaccUCGGCGcCGAgugCGCCGc -3' miRNA: 3'- caGCUGGCcu----AGCCGCaGCUa--GUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 35745 | 0.66 | 0.785266 |
Target: 5'- -gCGGCgGGAuuUCGuGUGUCGGgaagcgCGCCGa -3' miRNA: 3'- caGCUGgCCU--AGC-CGCAGCUa-----GUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 32175 | 0.66 | 0.785266 |
Target: 5'- uGUCGGCCGGGUCcaGCuucUCGAggaCGCCu -3' miRNA: 3'- -CAGCUGGCCUAGc-CGc--AGCUa--GUGGc -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 12183 | 0.66 | 0.785266 |
Target: 5'- cGUCuacCCGGAccCGGCGUCGGgcuuccccgcgCGCCGc -3' miRNA: 3'- -CAGcu-GGCCUa-GCCGCAGCUa----------GUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 51304 | 0.66 | 0.779592 |
Target: 5'- -aCGGCCGGggUGGCGUucuuggagagaagcaUGG-CGCCGa -3' miRNA: 3'- caGCUGGCCuaGCCGCA---------------GCUaGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 19450 | 0.66 | 0.775782 |
Target: 5'- gGUCGACCcGGUCGaacuCGUCGGUCucguCCu -3' miRNA: 3'- -CAGCUGGcCUAGCc---GCAGCUAGu---GGc -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 24713 | 0.66 | 0.775782 |
Target: 5'- -aCGugCcauGGGUCGGCaucgcaGUCGA-CACCGg -3' miRNA: 3'- caGCugG---CCUAGCCG------CAGCUaGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 54504 | 0.66 | 0.763254 |
Target: 5'- --aGGCCaagagcuggucccaGGAUCGGUGgcccgaGAUCACCGc -3' miRNA: 3'- cagCUGG--------------CCUAGCCGCag----CUAGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 47038 | 0.66 | 0.756422 |
Target: 5'- uGUCGcCCGaGUCGGCGgcc-UCACCGu -3' miRNA: 3'- -CAGCuGGCcUAGCCGCagcuAGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 59587 | 0.66 | 0.746563 |
Target: 5'- aGUCGACCuGAUCacucaGGCGgauggCG-UCACCa -3' miRNA: 3'- -CAGCUGGcCUAG-----CCGCa----GCuAGUGGc -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 25788 | 0.67 | 0.706177 |
Target: 5'- uUCgGAUCGGcUCGGCGaCGAagccauUCACCGc -3' miRNA: 3'- cAG-CUGGCCuAGCCGCaGCU------AGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 24450 | 0.67 | 0.695892 |
Target: 5'- cGUUGACCGGcuGUcCGGCaaCGAggCGCCGg -3' miRNA: 3'- -CAGCUGGCC--UA-GCCGcaGCUa-GUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 27316 | 0.67 | 0.695892 |
Target: 5'- -aCGGCCaGGAggGGCG-CGA-CGCCGg -3' miRNA: 3'- caGCUGG-CCUagCCGCaGCUaGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 9055 | 0.67 | 0.684514 |
Target: 5'- cGUCGGCCaGGAcacugucgcgUCGcugaaguauggccGCGUCG-UCACCGg -3' miRNA: 3'- -CAGCUGG-CCU----------AGC-------------CGCAGCuAGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 7331 | 0.67 | 0.675164 |
Target: 5'- -aUGGCCuGGAgaagcgCGGUGUCGccGUUACCGg -3' miRNA: 3'- caGCUGG-CCUa-----GCCGCAGC--UAGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 52620 | 0.67 | 0.675164 |
Target: 5'- cUCGGCCaaGUCGGUgagGUCGAacUCGCCGu -3' miRNA: 3'- cAGCUGGccUAGCCG---CAGCU--AGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 56211 | 0.68 | 0.654287 |
Target: 5'- aGUCGGuggcCUGGAcCGGCGUCGA-C-CCGa -3' miRNA: 3'- -CAGCU----GGCCUaGCCGCAGCUaGuGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 58465 | 0.68 | 0.654287 |
Target: 5'- uGUCGGuaaccuuuCCGuucuuGUCGGUGUUGGUCACCa -3' miRNA: 3'- -CAGCU--------GGCc----UAGCCGCAGCUAGUGGc -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 39955 | 0.68 | 0.643816 |
Target: 5'- aGUCGAuCCGcGcgcGUCGGCGcCGGgucaacUCACCGg -3' miRNA: 3'- -CAGCU-GGC-C---UAGCCGCaGCU------AGUGGC- -5' |
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18248 | 5' | -56.8 | NC_004681.1 | + | 9407 | 0.68 | 0.643816 |
Target: 5'- cGUUGACCGGGUUgccguaGGCGUUGccgaagaaGUUGCCGc -3' miRNA: 3'- -CAGCUGGCCUAG------CCGCAGC--------UAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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