Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18260 | 5' | -52 | NC_004681.1 | + | 69637 | 0.66 | 0.931198 |
Target: 5'- aCuuGCAGucGGGGCa---CGGCCGCCg -3' miRNA: 3'- -GuuUGUCucUCCCGagaaGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 8640 | 0.66 | 0.925554 |
Target: 5'- uCGAACcGAaGGGGUUCUggcCGAUgGCCg -3' miRNA: 3'- -GUUUGuCUcUCCCGAGAa--GCUGgUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 29618 | 0.66 | 0.919633 |
Target: 5'- uGAACucuGAuGGCUCgacgUCGACCACg -3' miRNA: 3'- gUUUGucuCUcCCGAGa---AGCUGGUGg -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 31429 | 0.66 | 0.919633 |
Target: 5'- aGGACGGAuugGAGccguaGGCUg--CGACCACCa -3' miRNA: 3'- gUUUGUCU---CUC-----CCGAgaaGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 40721 | 0.66 | 0.913436 |
Target: 5'- ----gGGAGAGGGC-----GGCCACCa -3' miRNA: 3'- guuugUCUCUCCCGagaagCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 75793 | 0.66 | 0.911524 |
Target: 5'- gCAGGuCAGAGGGGGUgggggugguugcggUCUcgaaaUCG-CCGCCu -3' miRNA: 3'- -GUUU-GUCUCUCCCG--------------AGA-----AGCuGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 56241 | 0.67 | 0.900224 |
Target: 5'- gCGAGCuGcaAGAGGGCgacacCUUCGA-CACCc -3' miRNA: 3'- -GUUUGuC--UCUCCCGa----GAAGCUgGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 67294 | 0.67 | 0.899535 |
Target: 5'- --uACGGGGAGGGCaucucgcgugaggUCgaacuccUCGACCugGCCg -3' miRNA: 3'- guuUGUCUCUCCCG-------------AGa------AGCUGG--UGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 7783 | 0.67 | 0.893214 |
Target: 5'- -uGACGGAcuuGGucgaCUUUUCGACCACCg -3' miRNA: 3'- guUUGUCUcu-CCc---GAGAAGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 9124 | 0.67 | 0.885939 |
Target: 5'- -uGGCGuuGcGGGCUCguaccCGACCGCCg -3' miRNA: 3'- guUUGUcuCuCCCGAGaa---GCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 58826 | 0.67 | 0.878405 |
Target: 5'- cCGGACAGAGGccaacaacCUCUUCGACCcgcACCa -3' miRNA: 3'- -GUUUGUCUCUccc-----GAGAAGCUGG---UGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 50326 | 0.68 | 0.845803 |
Target: 5'- aCAAGCc--GAGGGCgccauuggagCUggCGACCACCu -3' miRNA: 3'- -GUUUGucuCUCCCGa---------GAa-GCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 57927 | 0.68 | 0.837072 |
Target: 5'- gAGAgGGAGGGGccagccccGCUcCUUCG-CCACCu -3' miRNA: 3'- gUUUgUCUCUCC--------CGA-GAAGCuGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 59009 | 0.68 | 0.833518 |
Target: 5'- -cGugGGGGAGGGCgcccaccgcgaaUUUGACCACg -3' miRNA: 3'- guUugUCUCUCCCGag----------AAGCUGGUGg -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 18288 | 0.69 | 0.818973 |
Target: 5'- --cGCcGGGAGGcGCUCcagGACCGCCa -3' miRNA: 3'- guuUGuCUCUCC-CGAGaagCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 45778 | 0.69 | 0.809625 |
Target: 5'- uCAAAC-GAGcAGGGUcCaUCGACUACCu -3' miRNA: 3'- -GUUUGuCUC-UCCCGaGaAGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 23472 | 0.69 | 0.809625 |
Target: 5'- gCGAACguGGAGGcGGGCaggaggUUGACCACCu -3' miRNA: 3'- -GUUUG--UCUCU-CCCGaga---AGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 53358 | 0.69 | 0.790381 |
Target: 5'- -----cGGGAGGGCUauacccUUGGCCGCCu -3' miRNA: 3'- guuuguCUCUCCCGAga----AGCUGGUGG- -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 7489 | 0.7 | 0.769467 |
Target: 5'- -cGACGGAcuugggcGGGGGCuUCUucUCGGCCGCg -3' miRNA: 3'- guUUGUCU-------CUCCCG-AGA--AGCUGGUGg -5' |
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18260 | 5' | -52 | NC_004681.1 | + | 45174 | 0.7 | 0.760307 |
Target: 5'- --cGCGGcGAGGGC-C-UCGACCGCg -3' miRNA: 3'- guuUGUCuCUCCCGaGaAGCUGGUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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