Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18306 | 5' | -48.7 | NC_004681.1 | + | 69874 | 0.67 | 0.969779 |
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Target: 5'- --uACGUGcGCG-CGGCCGcUGGCAAa -3' miRNA: 3'- cauUGUACaUGCuGUCGGU-ACCGUUg -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 16858 | 0.67 | 0.971075 |
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Target: 5'- -cGACGUGU-CGACAGgCAggaccggagcgucaGGCAGCu -3' miRNA: 3'- caUUGUACAuGCUGUCgGUa-------------CCGUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 20273 | 0.67 | 0.972944 |
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Target: 5'- -cGACAaGUcgaucaccgaGCG-CAGCCggGGCAGCg -3' miRNA: 3'- caUUGUaCA----------UGCuGUCGGuaCCGUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 33436 | 0.67 | 0.972944 |
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Target: 5'- cGUGAUGUGcggGCGGguGCCcUGGaCGGCg -3' miRNA: 3'- -CAUUGUACa--UGCUguCGGuACC-GUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 54271 | 0.66 | 0.975862 |
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Target: 5'- ----gAUG-ACGACAGCCuuguugacgGUGGCGAUu -3' miRNA: 3'- cauugUACaUGCUGUCGG---------UACCGUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 14674 | 0.66 | 0.978285 |
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Target: 5'- --uGCcgGUgccgaagGCGACugcGGCCGUGGUGGCg -3' miRNA: 3'- cauUGuaCA-------UGCUG---UCGGUACCGUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 69169 | 0.66 | 0.980284 |
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Target: 5'- --cGCGUGUACGcaccacugaaaggcGCAGCCAUcGGUGucGCa -3' miRNA: 3'- cauUGUACAUGC--------------UGUCGGUA-CCGU--UG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 29740 | 0.66 | 0.980997 |
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Target: 5'- uUAGCGUGuUGCcauCGGUCGUGGuCGACg -3' miRNA: 3'- cAUUGUAC-AUGcu-GUCGGUACC-GUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 49148 | 0.66 | 0.980997 |
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Target: 5'- --cGCGUG-ACGAUGGCagcGGCGACa -3' miRNA: 3'- cauUGUACaUGCUGUCGguaCCGUUG- -5' |
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| 18306 | 5' | -48.7 | NC_004681.1 | + | 52958 | 0.66 | 0.985268 |
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Target: 5'- -cGACGUGgccUGGCAGgC-UGGCGGCa -3' miRNA: 3'- caUUGUACau-GCUGUCgGuACCGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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