Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18342 | 3' | -58.5 | NC_004681.1 | + | 30974 | 0.66 | 0.607499 |
Target: 5'- gGGUGUCGCggggcuccagCGGG-UCGGCGgGCGg -3' miRNA: 3'- -CUACAGCGgaa-------GCUCgAGCCGCgCGC- -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 52979 | 0.66 | 0.609602 |
Target: 5'- -uUGUCGCCg-CG-GCUCGgGCaCGCGg -3' miRNA: 3'- cuACAGCGGaaGCuCGAGC-CGcGCGC- -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 68994 | 0.66 | 0.630655 |
Target: 5'- cGAUGgucagCGCC-UCGAugGCgUCGGC-CGCGg -3' miRNA: 3'- -CUACa----GCGGaAGCU--CG-AGCCGcGCGC- -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 57902 | 0.66 | 0.630655 |
Target: 5'- ---uUCGCCaccUCG-GCggcgCGGCGCGUGg -3' miRNA: 3'- cuacAGCGGa--AGCuCGa---GCCGCGCGC- -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 9106 | 0.66 | 0.630655 |
Target: 5'- --cGcCGCUggcUCGGGCUCuGGCGuUGCGg -3' miRNA: 3'- cuaCaGCGGa--AGCUCGAG-CCGC-GCGC- -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 14580 | 0.66 | 0.630655 |
Target: 5'- --aGUCGCCUUCG-GCacCGGCaaGCu -3' miRNA: 3'- cuaCAGCGGAAGCuCGa-GCCGcgCGc -5' |
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18342 | 3' | -58.5 | NC_004681.1 | + | 14442 | 0.66 | 0.641187 |
Target: 5'- --gGUgGCC-UCGAuguuCUUGGCGCGCa -3' miRNA: 3'- cuaCAgCGGaAGCUc---GAGCCGCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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