miRNA display CGI


Results 21 - 25 of 25 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
18526 5' -55.2 NC_004681.1 + 48652 0.66 0.799078
Target:  5'- aGCAGGGCUGggUGCUGccguCGCGCUcgaccgugaaguauUGGc -3'
miRNA:   3'- -CGUCCUGAUgaACGACc---GUGCGG--------------ACC- -5'
18526 5' -55.2 NC_004681.1 + 5225 0.66 0.800968
Target:  5'- uGCAGGACUccuACUacuacgcgcccgcUGUgGGCAUGCaguUGGa -3'
miRNA:   3'- -CGUCCUGA---UGA-------------ACGaCCGUGCGg--ACC- -5'
18526 5' -55.2 NC_004681.1 + 68502 0.66 0.801911
Target:  5'- cGCcacuGAaugGCUcGC-GGCACGCCUGGg -3'
miRNA:   3'- -CGuc--CUga-UGAaCGaCCGUGCGGACC- -5'
18526 5' -55.2 NC_004681.1 + 20543 0.66 0.801911
Target:  5'- gGCGGcGACcacCUUGCugucgaUGGuCGCGCCUGa -3'
miRNA:   3'- -CGUC-CUGau-GAACG------ACC-GUGCGGACc -5'
18526 5' -55.2 NC_004681.1 + 37192 0.66 0.811243
Target:  5'- aCAGGGCUGCgcgUGUggaucGGCugGUucugaggccgCUGGg -3'
miRNA:   3'- cGUCCUGAUGa--ACGa----CCGugCG----------GACC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.