Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18543 | 5' | -58 | NC_004682.1 | + | 10404 | 0.66 | 0.538116 |
Target: 5'- aGGUAC-CGGCGucccguugguguUCCuGCACggGCUCa -3' miRNA: 3'- -CCGUGuGCCGCu-----------AGGuCGUGgaCGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 12212 | 0.66 | 0.529647 |
Target: 5'- --aACACGGCGGUCgaCGGCAacggucguccUCUGUUCg -3' miRNA: 3'- ccgUGUGCCGCUAG--GUCGU----------GGACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 34186 | 0.66 | 0.51913 |
Target: 5'- aGCGCAUGcCGcg-CAGCGCCUGCUg -3' miRNA: 3'- cCGUGUGCcGCuagGUCGUGGACGAg -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 34405 | 0.66 | 0.51913 |
Target: 5'- aGGCGUACGuGUGGUUCAGCAgcgggcguucccUCUGCUg -3' miRNA: 3'- -CCGUGUGC-CGCUAGGUCGU------------GGACGAg -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 14238 | 0.66 | 0.508697 |
Target: 5'- aGGCGCGuCGGUGGUCCAcGUcaugagacgagGCCagacaaGCUCg -3' miRNA: 3'- -CCGUGU-GCCGCUAGGU-CG-----------UGGa-----CGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 42638 | 0.66 | 0.498353 |
Target: 5'- cGGCACGCcacaagGGCGGUCCAGUcgUUGgaCu -3' miRNA: 3'- -CCGUGUG------CCGCUAGGUCGugGACgaG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 24996 | 0.66 | 0.498353 |
Target: 5'- gGGUACgACGcCGAcUCggCGGCAUCUGCUCg -3' miRNA: 3'- -CCGUG-UGCcGCU-AG--GUCGUGGACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 29906 | 0.67 | 0.487084 |
Target: 5'- cGCcgaACGCGcCGAUCCGGCgcaguuccuggauACCUGCUg -3' miRNA: 3'- cCG---UGUGCcGCUAGGUCG-------------UGGACGAg -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 15970 | 0.67 | 0.477955 |
Target: 5'- --aGCACGGCaGAUCC-GCaagGCCgGCUCa -3' miRNA: 3'- ccgUGUGCCG-CUAGGuCG---UGGaCGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 44727 | 0.67 | 0.471916 |
Target: 5'- aGGC-CGCGuaGCGggCCAGCAUCgucuucugccaacgGCUCa -3' miRNA: 3'- -CCGuGUGC--CGCuaGGUCGUGGa-------------CGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 35744 | 0.67 | 0.467911 |
Target: 5'- cGGCGCGCucaccggggaGGUGAUagcgcucguccaCCAGCGCCagGUUCc -3' miRNA: 3'- -CCGUGUG----------CCGCUA------------GGUCGUGGa-CGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 9388 | 0.67 | 0.457977 |
Target: 5'- --gGCGCGGuCGA-CgAGCGCCUGCg- -3' miRNA: 3'- ccgUGUGCC-GCUaGgUCGUGGACGag -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 5817 | 0.67 | 0.447182 |
Target: 5'- cGGCaacaucuggaacgACGCGGUGAcCCGcGCcuACCUGUUCg -3' miRNA: 3'- -CCG-------------UGUGCCGCUaGGU-CG--UGGACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 20057 | 0.68 | 0.428874 |
Target: 5'- aGGCucGCACGGCucgcaugucGAUcaCCGGCAUCccgGCUCa -3' miRNA: 3'- -CCG--UGUGCCG---------CUA--GGUCGUGGa--CGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 14611 | 0.68 | 0.410089 |
Target: 5'- cGGUggGCACGGCuGcgCCGaCuCCUGCUCa -3' miRNA: 3'- -CCG--UGUGCCG-CuaGGUcGuGGACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 10071 | 0.68 | 0.40089 |
Target: 5'- aGGCACAgGGCcuGggCCucaugGGCACCaUGUUCg -3' miRNA: 3'- -CCGUGUgCCG--CuaGG-----UCGUGG-ACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 24155 | 0.68 | 0.390924 |
Target: 5'- gGGCAa--GGCGAUCCuggaggacauggcGGCGaagCUGCUCg -3' miRNA: 3'- -CCGUgugCCGCUAGG-------------UCGUg--GACGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 3170 | 0.68 | 0.382892 |
Target: 5'- gGGCAcCACGGCGggCCAGgGCgCggGCa- -3' miRNA: 3'- -CCGU-GUGCCGCuaGGUCgUG-Ga-CGag -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 21187 | 0.69 | 0.373225 |
Target: 5'- cGGCGCuaccuccACGGCGAagaCCcGCACCcGUUCg -3' miRNA: 3'- -CCGUG-------UGCCGCUa--GGuCGUGGaCGAG- -5' |
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18543 | 5' | -58 | NC_004682.1 | + | 17704 | 0.69 | 0.365439 |
Target: 5'- cGGCGCuGCaGCaGAUCCAGCcgaugauccccgGCCUGgUCg -3' miRNA: 3'- -CCGUG-UGcCG-CUAGGUCG------------UGGACgAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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