Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18556 | 3' | -54.2 | NC_004682.1 | + | 1046 | 0.73 | 0.379991 |
Target: 5'- cGCUACUaCGAGCCGuccgacuggcaguacGCcCGGACAGCg -3' miRNA: 3'- -UGGUGA-GCUUGGCuu-------------CGaGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 2944 | 0.71 | 0.439096 |
Target: 5'- aACCugaUCGAcuuCUGggGCgCGGGCGGCg -3' miRNA: 3'- -UGGug-AGCUu--GGCuuCGaGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 3173 | 0.7 | 0.531776 |
Target: 5'- cACCACggCGGGCCaGGGCgCGGGCAaccGCg -3' miRNA: 3'- -UGGUGa-GCUUGGcUUCGaGCCUGU---CG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 4734 | 0.66 | 0.78026 |
Target: 5'- gGCCACgaaguggaUCGAguaccacGCCGAAGCcgacgacugCGGAgacCAGCa -3' miRNA: 3'- -UGGUG--------AGCU-------UGGCUUCGa--------GCCU---GUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 4875 | 0.77 | 0.202847 |
Target: 5'- aGCCACgCGGAgUGAagGGCUCGGugAGCc -3' miRNA: 3'- -UGGUGaGCUUgGCU--UCGAGCCugUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 4949 | 0.69 | 0.608374 |
Target: 5'- gGCUACgaCGAcacccCCGguGCUCGGGCGGUc -3' miRNA: 3'- -UGGUGa-GCUu----GGCuuCGAGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 5417 | 0.66 | 0.739964 |
Target: 5'- gACCACucaUCGAGCgGAGGUgaggCGcGAaaCAGCa -3' miRNA: 3'- -UGGUG---AGCUUGgCUUCGa---GC-CU--GUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 5864 | 0.75 | 0.295471 |
Target: 5'- uGCCACuggcuguugaguacgUCGAGCgCGuuGCUCGcGACGGCg -3' miRNA: 3'- -UGGUG---------------AGCUUG-GCuuCGAGC-CUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 6334 | 0.67 | 0.717523 |
Target: 5'- cCCGCUgGGuccgggacuccccGCCGAcacGGCgCGGGCGGUg -3' miRNA: 3'- uGGUGAgCU-------------UGGCU---UCGaGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 6857 | 0.67 | 0.674922 |
Target: 5'- gGCCcgaACUcCGuugugucccAACUGAucgAGCUUGGACAGCg -3' miRNA: 3'- -UGG---UGA-GC---------UUGGCU---UCGAGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 7285 | 0.68 | 0.630579 |
Target: 5'- gGCUACaUCGAcACCGu-GCUgagGGACAGCg -3' miRNA: 3'- -UGGUG-AGCU-UGGCuuCGAg--CCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 7696 | 0.74 | 0.315342 |
Target: 5'- gACCGCcUGGACCGGAGCgUGG-CGGCu -3' miRNA: 3'- -UGGUGaGCUUGGCUUCGaGCCuGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 8058 | 0.68 | 0.630579 |
Target: 5'- uCCACaUgGGACCGAAGCcCGGuCuGCc -3' miRNA: 3'- uGGUG-AgCUUGGCUUCGaGCCuGuCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 9436 | 0.67 | 0.674922 |
Target: 5'- uUCGCUCGGucACCGAcgcAGCUgccCGGAC-GCu -3' miRNA: 3'- uGGUGAGCU--UGGCU---UCGA---GCCUGuCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 11006 | 0.75 | 0.270974 |
Target: 5'- cGCgGCUggcaagagCGcAGCCGAGGCaUCGGGCGGCg -3' miRNA: 3'- -UGgUGA--------GC-UUGGCUUCG-AGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 11509 | 0.68 | 0.630579 |
Target: 5'- cCCACUCa-----GGGCUCGGGCAGCc -3' miRNA: 3'- uGGUGAGcuuggcUUCGAGCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 14670 | 0.8 | 0.138032 |
Target: 5'- gGCCugUgGAcGCCGAccGGCUgGGACAGCa -3' miRNA: 3'- -UGGugAgCU-UGGCU--UCGAgCCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 15238 | 0.66 | 0.778245 |
Target: 5'- aGCC-CUCGGACCacuucGAguucacguucaugcGGCUCauggaGGGCGGCg -3' miRNA: 3'- -UGGuGAGCUUGG-----CU--------------UCGAG-----CCUGUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 15317 | 0.71 | 0.479233 |
Target: 5'- cGCCugUCaAGCUGGAGCUgGGA-GGCg -3' miRNA: 3'- -UGGugAGcUUGGCUUCGAgCCUgUCG- -5' |
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18556 | 3' | -54.2 | NC_004682.1 | + | 15944 | 0.72 | 0.410287 |
Target: 5'- cGCCAacggCGAGCCGggGUUCuGGACucgucGCu -3' miRNA: 3'- -UGGUga--GCUUGGCuuCGAG-CCUGu----CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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