Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18620 | 5' | -58.7 | NC_004682.1 | + | 14905 | 0.7 | 0.339699 |
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Target: 5'- uCGGcguCUCGGCUGcgagcggcgacccGACCGAGaaGGCGUUCc -3' miRNA: 3'- -GCUu--GAGCCGAC-------------CUGGCUC--CCGCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 34229 | 0.7 | 0.324832 |
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Target: 5'- uCGAucacCUCGGCga---CGAGGGUGUCCu -3' miRNA: 3'- -GCUu---GAGCCGaccugGCUCCCGCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 35603 | 0.72 | 0.254614 |
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Target: 5'- uGAACUCGaagUGGuCCGAGGGCuucucgGUCCa -3' miRNA: 3'- gCUUGAGCcg-ACCuGGCUCCCG------CAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 22980 | 0.73 | 0.207824 |
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Target: 5'- cCGGGCUggccuucgGGCUGGuCCGGGGGgcCGUCCu -3' miRNA: 3'- -GCUUGAg-------CCGACCuGGCUCCC--GCAGG- -5' |
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| 18620 | 5' | -58.7 | NC_004682.1 | + | 12662 | 1.12 | 0.000286 |
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Target: 5'- aCGAACUCGGCUGGACCGAGGGCGUCCa -3' miRNA: 3'- -GCUUGAGCCGACCUGGCUCCCGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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