Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18720 | 5' | -60.4 | NC_004682.1 | + | 44245 | 0.66 | 0.52541 |
Target: 5'- aCCCACGAucCCGUCgucguCUCCGACCccCGa-- -3' miRNA: 3'- -GGGUGCU--GGCAG-----GAGGCUGGa-GCccc -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 455 | 0.66 | 0.52541 |
Target: 5'- cUCCAgGGCCGguaUCCagCGGCCUCcaGGGc -3' miRNA: 3'- -GGGUgCUGGC---AGGagGCUGGAGc-CCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 4856 | 0.66 | 0.505567 |
Target: 5'- uCCCACaGCCgGUCCuUCUGAgCCUgCGuGGGc -3' miRNA: 3'- -GGGUGcUGG-CAGG-AGGCU-GGA-GC-CCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 7685 | 0.66 | 0.495766 |
Target: 5'- aCguCGGgCGguuaCCUUCGACCgaugCGGGGg -3' miRNA: 3'- gGguGCUgGCa---GGAGGCUGGa---GCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 36402 | 0.66 | 0.495766 |
Target: 5'- uCCCACGACgaagcgCGUCCUCCucgGACuugguCUCGGcGa -3' miRNA: 3'- -GGGUGCUG------GCAGGAGG---CUG-----GAGCC-Cc -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 13178 | 0.66 | 0.49479 |
Target: 5'- -gUACGuguaguuGCCGUCgguCUCCGAcagguagcCCUCGGGGu -3' miRNA: 3'- ggGUGC-------UGGCAG---GAGGCU--------GGAGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 21067 | 0.66 | 0.476429 |
Target: 5'- gUCAa-GCCGUUC-CCGcuCCUCGGGGa -3' miRNA: 3'- gGGUgcUGGCAGGaGGCu-GGAGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 15670 | 0.66 | 0.476429 |
Target: 5'- aCgGCGACUG-CCUagucgCUGACCUCuGGGa -3' miRNA: 3'- gGgUGCUGGCaGGA-----GGCUGGAGcCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 26097 | 0.67 | 0.466902 |
Target: 5'- gCCUACGGCgggaGUCCgacgCCGGCCUCa--- -3' miRNA: 3'- -GGGUGCUGg---CAGGa---GGCUGGAGcccc -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 49959 | 0.67 | 0.424409 |
Target: 5'- gCCUGUGGCCGUCCcgaugagcaugcugcUCCGGuugacaacCCUUGGGGc -3' miRNA: 3'- -GGGUGCUGGCAGG---------------AGGCU--------GGAGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 47203 | 0.67 | 0.420824 |
Target: 5'- aCUCACGGuuGgcugCCUgcaugaacUCGaACCUCGGGGu -3' miRNA: 3'- -GGGUGCUggCa---GGA--------GGC-UGGAGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 9378 | 0.67 | 0.419931 |
Target: 5'- aCCCACuGGCCGUCCUCgcgguuccagaUGACCguguuguUCGGcaGGu -3' miRNA: 3'- -GGGUG-CUGGCAGGAG-----------GCUGG-------AGCC--CC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 23648 | 0.68 | 0.403176 |
Target: 5'- aCCC-UGACCGacgggCCUgCGGCCcuccugCGGGGc -3' miRNA: 3'- -GGGuGCUGGCa----GGAgGCUGGa-----GCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 13514 | 0.68 | 0.403176 |
Target: 5'- gUCugGguguuGCCGUCCUCgucggugaCGACCucuUCGGGGu -3' miRNA: 3'- gGGugC-----UGGCAGGAG--------GCUGG---AGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 14127 | 0.68 | 0.369321 |
Target: 5'- cCCCGCGuccaggccgUCGUCCUcCCGAuCCUCcGGGc -3' miRNA: 3'- -GGGUGCu--------GGCAGGA-GGCU-GGAGcCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 48933 | 0.69 | 0.356335 |
Target: 5'- gCCGCGACCaUCuCUCCGAgcaugaugaucauguCUUCGcGGGa -3' miRNA: 3'- gGGUGCUGGcAG-GAGGCU---------------GGAGC-CCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 32161 | 0.69 | 0.353139 |
Target: 5'- cCCCGCaACCGUgUCUCgGAUCgucCGGGGc -3' miRNA: 3'- -GGGUGcUGGCA-GGAGgCUGGa--GCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 35393 | 0.69 | 0.327552 |
Target: 5'- cCUCGCGGgaGUCCUcgaugacgaaggcaCCGGCCcagUCGGGGu -3' miRNA: 3'- -GGGUGCUggCAGGA--------------GGCUGG---AGCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 43410 | 0.69 | 0.322304 |
Target: 5'- uUCC-CGGCCauguaguaGUCC-CCGAUCUgCGGGGg -3' miRNA: 3'- -GGGuGCUGG--------CAGGaGGCUGGA-GCCCC- -5' |
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18720 | 5' | -60.4 | NC_004682.1 | + | 19865 | 0.7 | 0.279904 |
Target: 5'- gUCCGgGACCGUCCa--GAUCgucgCGGGGg -3' miRNA: 3'- -GGGUgCUGGCAGGaggCUGGa---GCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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