Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18750 | 3' | -56.1 | NC_004683.1 | + | 51713 | 0.69 | 0.473355 |
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Target: 5'- gGCAGCGCGaccgCGGGCuCGGGggCggGCu -3' miRNA: 3'- -UGUCGCGCaa--GUCCG-GCUCaaGgaCG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 51813 | 0.75 | 0.202185 |
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Target: 5'- gGCGGCGCGgcacuggUCGcGGCCGuAGcacUCCUGCa -3' miRNA: 3'- -UGUCGCGCa------AGU-CCGGC-UCa--AGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 52126 | 0.67 | 0.589375 |
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Target: 5'- -gGGCGCG-UCGGGCaCG-GUUCCcaGCc -3' miRNA: 3'- ugUCGCGCaAGUCCG-GCuCAAGGa-CG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 53202 | 0.66 | 0.676902 |
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Target: 5'- uGCAGCGCG--CAcGCCGugagcacgacgAGUUCCUGa -3' miRNA: 3'- -UGUCGCGCaaGUcCGGC-----------UCAAGGACg -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 53624 | 0.68 | 0.522825 |
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Target: 5'- uGCGGCGCGaUCAGGCCcgccgcgaCCUGg -3' miRNA: 3'- -UGUCGCGCaAGUCCGGcucaa---GGACg -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 54430 | 0.68 | 0.53233 |
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Target: 5'- uGCAGCGCuUUCAuGGCCcgcucaaccugcgcGGGUUCCcaagUGCc -3' miRNA: 3'- -UGUCGCGcAAGU-CCGG--------------CUCAAGG----ACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 56321 | 0.68 | 0.556887 |
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Target: 5'- gUAGCGCGUUCucgauGUCGGGUUgCCaGCg -3' miRNA: 3'- uGUCGCGCAAGuc---CGGCUCAA-GGaCG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 56575 | 0.67 | 0.622183 |
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Target: 5'- uGCGGUGCGgcCGGGCaugcguCGGGa-CCUGCg -3' miRNA: 3'- -UGUCGCGCaaGUCCG------GCUCaaGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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