Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18750 | 3' | -56.1 | NC_004683.1 | + | 18982 | 0.69 | 0.504019 |
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Target: 5'- gACGGUGCGcacgaacuccCGGGCCGcc-UCCUGCg -3' miRNA: 3'- -UGUCGCGCaa--------GUCCGGCucaAGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 400 | 0.69 | 0.493698 |
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Target: 5'- cGCAGCGCcugCuGGCCGAGaaccgucUCCaUGCg -3' miRNA: 3'- -UGUCGCGcaaGuCCGGCUCa------AGG-ACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 8625 | 0.69 | 0.493698 |
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Target: 5'- cACGGCGa--UCAGGCCGgugcGGUggccgcCCUGCa -3' miRNA: 3'- -UGUCGCgcaAGUCCGGC----UCAa-----GGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 51713 | 0.69 | 0.473355 |
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Target: 5'- gGCAGCGCGaccgCGGGCuCGGGggCggGCu -3' miRNA: 3'- -UGUCGCGCaa--GUCCG-GCUCaaGgaCG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 4526 | 0.7 | 0.443657 |
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Target: 5'- cGCuGCGCGUcgUCGgacucGGCCGGGUguucaaCUGCg -3' miRNA: 3'- -UGuCGCGCA--AGU-----CCGGCUCAag----GACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 51813 | 0.75 | 0.202185 |
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Target: 5'- gGCGGCGCGgcacuggUCGcGGCCGuAGcacUCCUGCa -3' miRNA: 3'- -UGUCGCGCa------AGU-CCGGC-UCa--AGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 692 | 1.11 | 0.000599 |
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Target: 5'- aACAGCGCGUUCAGGCCGAGUUCCUGCu -3' miRNA: 3'- -UGUCGCGCAAGUCCGGCUCAAGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 27541 | 0.66 | 0.643018 |
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Target: 5'- cCAGCGCGcUCGcugcGGCUgccugcggugugaGGGUUCCUGg -3' miRNA: 3'- uGUCGCGCaAGU----CCGG-------------CUCAAGGACg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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