Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18750 | 3' | -56.1 | NC_004683.1 | + | 56575 | 0.67 | 0.622183 |
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Target: 5'- uGCGGUGCGgcCGGGCaugcguCGGGa-CCUGCg -3' miRNA: 3'- -UGUCGCGCaaGUCCG------GCUCaaGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 33341 | 0.67 | 0.622183 |
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Target: 5'- gGCgAGCGCGUcgCGGGCCucuuucucggugGGGUaccgUCUGCg -3' miRNA: 3'- -UG-UCGCGCAa-GUCCGG------------CUCAa---GGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 24640 | 0.67 | 0.633149 |
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Target: 5'- cCGGCGCc--CAGGCCgccGAGUUcgcgcgCCUGCu -3' miRNA: 3'- uGUCGCGcaaGUCCGG---CUCAA------GGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 24166 | 0.67 | 0.637536 |
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Target: 5'- aGCAGCGCGggCcagcccuuggccugcGGGCCGAacagCUGCu -3' miRNA: 3'- -UGUCGCGCaaG---------------UCCGGCUcaagGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 27541 | 0.66 | 0.643018 |
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Target: 5'- cCAGCGCGcUCGcugcGGCUgccugcggugugaGGGUUCCUGg -3' miRNA: 3'- uGUCGCGCaAGU----CCGG-------------CUCAAGGACg -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 21946 | 0.66 | 0.644114 |
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Target: 5'- gGCGGUgGCGggCGGccGCCGAGUUCaccgGCc -3' miRNA: 3'- -UGUCG-CGCaaGUC--CGGCUCAAGga--CG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 53202 | 0.66 | 0.676902 |
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Target: 5'- uGCAGCGCG--CAcGCCGugagcacgacgAGUUCCUGa -3' miRNA: 3'- -UGUCGCGCaaGUcCGGC-----------UCAAGGACg -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 27219 | 0.66 | 0.676902 |
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Target: 5'- -uGGCGCGUcUCGaugccGCCGAGcgauucgaUCCUGCg -3' miRNA: 3'- ugUCGCGCA-AGUc----CGGCUCa-------AGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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