Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18750 | 3' | -56.1 | NC_004683.1 | + | 51713 | 0.69 | 0.473355 |
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Target: 5'- gGCAGCGCGaccgCGGGCuCGGGggCggGCu -3' miRNA: 3'- -UGUCGCGCaa--GUCCG-GCUCaaGgaCG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 4526 | 0.7 | 0.443657 |
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Target: 5'- cGCuGCGCGUcgUCGgacucGGCCGGGUguucaaCUGCg -3' miRNA: 3'- -UGuCGCGCA--AGU-----CCGGCUCAag----GACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 10193 | 0.72 | 0.312674 |
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Target: 5'- -aGGCGCGUgagCGGGCCGAGgaacaggCC-GCc -3' miRNA: 3'- ugUCGCGCAa--GUCCGGCUCaa-----GGaCG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 25996 | 0.74 | 0.262382 |
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Target: 5'- cGCGGCGaucucgCuGGCCGcGUUCCUGCg -3' miRNA: 3'- -UGUCGCgcaa--GuCCGGCuCAAGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 47596 | 0.75 | 0.230632 |
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Target: 5'- -uGGCgGCGUUCGGGCCGA--UgCUGCa -3' miRNA: 3'- ugUCG-CGCAAGUCCGGCUcaAgGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 51813 | 0.75 | 0.202185 |
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Target: 5'- gGCGGCGCGgcacuggUCGcGGCCGuAGcacUCCUGCa -3' miRNA: 3'- -UGUCGCGCa------AGU-CCGGC-UCa--AGGACG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 4957 | 0.77 | 0.167511 |
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Target: 5'- cGCAuGCGCGUcgAGGCCG-GUUCCUcGCa -3' miRNA: 3'- -UGU-CGCGCAagUCCGGCuCAAGGA-CG- -5' |
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| 18750 | 3' | -56.1 | NC_004683.1 | + | 692 | 1.11 | 0.000599 |
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Target: 5'- aACAGCGCGUUCAGGCCGAGUUCCUGCu -3' miRNA: 3'- -UGUCGCGCAAGUCCGGCUCAAGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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