Results 1 - 20 of 37 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18750 | 5' | -60.3 | NC_004683.1 | + | 18082 | 0.66 | 0.494312 |
Target: 5'- cCGGCGGGcACCaCgGCCAccuaucAGaUCgGCACGa -3' miRNA: 3'- -GCCGCCCuUGG-GgCGGU------UC-AG-CGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 48228 | 0.66 | 0.493319 |
Target: 5'- gCGGCGGGAacaaaucgcGCCgaaaugagcgacgCCGCCGgcgAGUggcUGCGCAg -3' miRNA: 3'- -GCCGCCCU---------UGG-------------GGCGGU---UCA---GCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 14564 | 0.66 | 0.484426 |
Target: 5'- gCGGUGGGuucaucuACgCCGaCGGGUCGCAg- -3' miRNA: 3'- -GCCGCCCu------UGgGGCgGUUCAGCGUgu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 35791 | 0.66 | 0.481478 |
Target: 5'- aGGUGGucaaaccguuaucgGggUCCCGCCAGGcUCagGCGCGu -3' miRNA: 3'- gCCGCC--------------CuuGGGGCGGUUC-AG--CGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 32457 | 0.66 | 0.474635 |
Target: 5'- -uGUGGGcACCCCGCgGAGaUGCAUc -3' miRNA: 3'- gcCGCCCuUGGGGCGgUUCaGCGUGu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 56711 | 0.66 | 0.474635 |
Target: 5'- aCGGacuGAGCCCCGaCGAGgCGCGCAu -3' miRNA: 3'- -GCCgccCUUGGGGCgGUUCaGCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 55222 | 0.66 | 0.455357 |
Target: 5'- cCGaGCGGGuGACCUCGCCAuGcUCGUcCAu -3' miRNA: 3'- -GC-CGCCC-UUGGGGCGGUuC-AGCGuGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 5829 | 0.66 | 0.455357 |
Target: 5'- uCGGCGGcGGugCCCGC-GAG-CGCGg- -3' miRNA: 3'- -GCCGCC-CUugGGGCGgUUCaGCGUgu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 585 | 0.66 | 0.455357 |
Target: 5'- uGGCGGGGuUCCCGCCGGaUCccgaGCAg -3' miRNA: 3'- gCCGCCCUuGGGGCGGUUcAGcg--UGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 24251 | 0.67 | 0.41812 |
Target: 5'- gCGGUGGGcccgcGCCUaCGCCGAGUCcUACGc -3' miRNA: 3'- -GCCGCCCu----UGGG-GCGGUUCAGcGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 9838 | 0.67 | 0.41812 |
Target: 5'- cCGGgGGcGAAUCCCGCUuuGAGgaacuuggCGUACAc -3' miRNA: 3'- -GCCgCC-CUUGGGGCGG--UUCa-------GCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 7844 | 0.67 | 0.415402 |
Target: 5'- aCGGCGaguuccgccgugucGGAcccgacACCCCGaCCAAGgcgcagggcaUCGCGCAg -3' miRNA: 3'- -GCCGC--------------CCU------UGGGGC-GGUUC----------AGCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 44861 | 0.67 | 0.409103 |
Target: 5'- -cGCGGGcacgcggcccGCCgCCGCCGGGUCGUAg- -3' miRNA: 3'- gcCGCCCu---------UGG-GGCGGUUCAGCGUgu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 51547 | 0.67 | 0.409103 |
Target: 5'- aCGGCaGGcGCgCCGCCAg--CGCACGc -3' miRNA: 3'- -GCCGcCCuUGgGGCGGUucaGCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 50932 | 0.67 | 0.405531 |
Target: 5'- uGGgGGGAGaCCCGCaaaaccuucccGUCGCGCAc -3' miRNA: 3'- gCCgCCCUUgGGGCGguu--------CAGCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 1423 | 0.67 | 0.400209 |
Target: 5'- uCGGCGGGA---UCGagCGAGUCGCGCu -3' miRNA: 3'- -GCCGCCCUuggGGCg-GUUCAGCGUGu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 44358 | 0.67 | 0.400209 |
Target: 5'- uCGGCGcGGAucGCCgCGUCGAGcCGCGu- -3' miRNA: 3'- -GCCGC-CCU--UGGgGCGGUUCaGCGUgu -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 9725 | 0.67 | 0.400209 |
Target: 5'- aGGauGGGACCCUGCCcGAGgUGUACGc -3' miRNA: 3'- gCCgcCCUUGGGGCGG-UUCaGCGUGU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 12236 | 0.67 | 0.400209 |
Target: 5'- gGGCGGGAGCgCCCggcgugccgauGCCcGG-CGCugGu -3' miRNA: 3'- gCCGCCCUUG-GGG-----------CGGuUCaGCGugU- -5' |
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18750 | 5' | -60.3 | NC_004683.1 | + | 46057 | 0.67 | 0.391439 |
Target: 5'- -uGUGGGAcguGCCCCgguucGCCGAGgCGCACc -3' miRNA: 3'- gcCGCCCU---UGGGG-----CGGUUCaGCGUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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