Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18751 | 3' | -52.4 | NC_004683.1 | + | 46809 | 0.68 | 0.770147 |
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Target: 5'- -gUGGUGCGCGUCg-GAaucGGAUCGucagugcucguCGCg -3' miRNA: 3'- caACCACGCGCGGaaCU---UCUAGU-----------GCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 47615 | 0.68 | 0.770147 |
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Target: 5'- --cGGUcGUGCaGCCa--GGGAUCACGCa -3' miRNA: 3'- caaCCA-CGCG-CGGaacUUCUAGUGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 51173 | 0.68 | 0.727697 |
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Target: 5'- -cUGGauccgGCGCGCCgccGAGAaCGCGCc -3' miRNA: 3'- caACCa----CGCGCGGaacUUCUaGUGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 51548 | 0.68 | 0.759727 |
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Target: 5'- --cGGcagGCGCGCCgcc-AGcgCACGCa -3' miRNA: 3'- caaCCa--CGCGCGGaacuUCuaGUGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 51621 | 0.69 | 0.694768 |
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Target: 5'- -cUGGcgGCGCGCCUgccgugcgUGAuGGAUCAgGUc -3' miRNA: 3'- caACCa-CGCGCGGA--------ACU-UCUAGUgCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 52323 | 0.69 | 0.716805 |
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Target: 5'- cUUGGUGcCGCGCCggcccGGGUCGCcgagGCg -3' miRNA: 3'- cAACCAC-GCGCGGaacu-UCUAGUG----CG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 54330 | 0.68 | 0.727697 |
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Target: 5'- --cGGUGgGCauguuaggugGCa-UGggGAUCACGCg -3' miRNA: 3'- caaCCACgCG----------CGgaACuuCUAGUGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 55044 | 0.68 | 0.759727 |
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Target: 5'- --cGGUGUGCGU-----AGAUCAUGCa -3' miRNA: 3'- caaCCACGCGCGgaacuUCUAGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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