Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18751 | 3' | -52.4 | NC_004683.1 | + | 6841 | 0.67 | 0.819772 |
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Target: 5'- --cGGU-CGCGCCgc-AAGggCACGCu -3' miRNA: 3'- caaCCAcGCGCGGaacUUCuaGUGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 5788 | 0.66 | 0.829122 |
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Target: 5'- -cUGGuUGCccuggccgGUGCCggucgUGAGGGUCACGg -3' miRNA: 3'- caACC-ACG--------CGCGGa----ACUUCUAGUGCg -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 16559 | 0.66 | 0.831885 |
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Target: 5'- gGUUGGUGCgcaccacguacauggGCGUCUUGAGcAUCuccagcaGCGCc -3' miRNA: 3'- -CAACCACG---------------CGCGGAACUUcUAG-------UGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 43975 | 0.66 | 0.838255 |
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Target: 5'- -----cGCGCGCCUcccGGAUCgACGCg -3' miRNA: 3'- caaccaCGCGCGGAacuUCUAG-UGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 30463 | 0.66 | 0.838255 |
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Target: 5'- --cGGUuacUGCGCCUaGAuGAUCugGCu -3' miRNA: 3'- caaCCAc--GCGCGGAaCUuCUAGugCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 40092 | 0.66 | 0.855831 |
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Target: 5'- --cGGgcccaGCGCGCCUUGAcAGAccagCcggACGCg -3' miRNA: 3'- caaCCa----CGCGCGGAACU-UCUa---G---UGCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 14409 | 0.66 | 0.864256 |
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Target: 5'- cUUGGUccuGCGCGCCgacaacGGGuUCugGCg -3' miRNA: 3'- cAACCA---CGCGCGGaac---UUCuAGugCG- -5' |
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| 18751 | 3' | -52.4 | NC_004683.1 | + | 3456 | 0.66 | 0.872428 |
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Target: 5'- --aGGUcCGCGCCgaGAcGAUCaacACGCu -3' miRNA: 3'- caaCCAcGCGCGGaaCUuCUAG---UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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