Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18758 | 3' | -57.2 | NC_004683.1 | + | 13366 | 0.66 | 0.657032 |
Target: 5'- -gUUCGUguucGCCGGGCUGuaGAuauCCGCUg -3' miRNA: 3'- cuGAGCAa---CGGCCUGACcgCU---GGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 20115 | 0.66 | 0.646265 |
Target: 5'- cGC-CGUaucaCCGGuCUGGCcgcGACCACCa -3' miRNA: 3'- cUGaGCAac--GGCCuGACCG---CUGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 7402 | 0.66 | 0.646265 |
Target: 5'- gGAUUCGgcgGCCGGGC-GcGCuGCCACa -3' miRNA: 3'- -CUGAGCaa-CGGCCUGaC-CGcUGGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 31386 | 0.66 | 0.635486 |
Target: 5'- cGAUguggCGguagGCCaGcACUgcGGCGACCGCCa -3' miRNA: 3'- -CUGa---GCaa--CGGcC-UGA--CCGCUGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 26792 | 0.66 | 0.635486 |
Target: 5'- cACUCuUUGUCGcGcagggcuauccGCUGGCGgGCCACCu -3' miRNA: 3'- cUGAGcAACGGC-C-----------UGACCGC-UGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 48608 | 0.66 | 0.622547 |
Target: 5'- uGACgg--UGCCGGcgaggacACUGGCGcggcauugaucgcACCGCCa -3' miRNA: 3'- -CUGagcaACGGCC-------UGACCGC-------------UGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 3493 | 0.66 | 0.613927 |
Target: 5'- gGGCUaCGa-GCCGGAaucggUGGUGGCCGCg -3' miRNA: 3'- -CUGA-GCaaCGGCCUg----ACCGCUGGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 25878 | 0.66 | 0.601017 |
Target: 5'- cGGCUCGgcgucgaguucGCCGGGCagacGCuGGCCGCCu -3' miRNA: 3'- -CUGAGCaa---------CGGCCUGac--CG-CUGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 42588 | 0.67 | 0.591357 |
Target: 5'- cGGCggUCGggGCCGGucguccgGCUgggguggucGGCGACCGCg -3' miRNA: 3'- -CUG--AGCaaCGGCC-------UGA---------CCGCUGGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 45152 | 0.67 | 0.581724 |
Target: 5'- -uCUCa--GCaCGGGCUGGCcGACCGCa -3' miRNA: 3'- cuGAGcaaCG-GCCUGACCG-CUGGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 51289 | 0.67 | 0.581724 |
Target: 5'- cACUCaucGCCGaGCUGGagGGCCGCCg -3' miRNA: 3'- cUGAGcaaCGGCcUGACCg-CUGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 25281 | 0.67 | 0.581724 |
Target: 5'- uGGCcgCGcUGCCGGGCcugcaGGCGcacagcgaguACCACCu -3' miRNA: 3'- -CUGa-GCaACGGCCUGa----CCGC----------UGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 10803 | 0.67 | 0.57532 |
Target: 5'- cGGCUCGgaucagcgggaggGCCaaucGGAUUGGCGcuggccggugugGCCACCc -3' miRNA: 3'- -CUGAGCaa-----------CGG----CCUGACCGC------------UGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 52676 | 0.67 | 0.57106 |
Target: 5'- uACUCGggugGCuCGGGCcGGuUGACCACg -3' miRNA: 3'- cUGAGCaa--CG-GCCUGaCC-GCUGGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 9271 | 0.67 | 0.549881 |
Target: 5'- -cCUCGcggggcgcaUGcCCGGGCUGGaCGugCGCCc -3' miRNA: 3'- cuGAGCa--------AC-GGCCUGACC-GCugGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 24354 | 0.68 | 0.539382 |
Target: 5'- cGACUCGUcGCCGcaGGCcacgcggucGCGAUCACCg -3' miRNA: 3'- -CUGAGCAaCGGC--CUGac-------CGCUGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 30871 | 0.68 | 0.528951 |
Target: 5'- uGACggCGUU-CCGGGC-GGCGugCGCa -3' miRNA: 3'- -CUGa-GCAAcGGCCUGaCCGCugGUGg -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 51637 | 0.68 | 0.528951 |
Target: 5'- -cCUCGUucUGCgUGcGCUGGCGGCgCGCCu -3' miRNA: 3'- cuGAGCA--ACG-GCcUGACCGCUG-GUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 42729 | 0.68 | 0.527912 |
Target: 5'- gGGCUCGcUGUCGGuauCcGGCGcgaagcugcuguuGCCGCCg -3' miRNA: 3'- -CUGAGCaACGGCCu--GaCCGC-------------UGGUGG- -5' |
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18758 | 3' | -57.2 | NC_004683.1 | + | 47079 | 0.68 | 0.508322 |
Target: 5'- cGGCUCGguaCCGGugUGGacaucugccccUGAUCGCCa -3' miRNA: 3'- -CUGAGCaacGGCCugACC-----------GCUGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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