Results 1 - 20 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18759 | 3' | -53.5 | NC_004683.1 | + | 38892 | 0.66 | 0.869379 |
Target: 5'- cCCGAuGAGCUGGGgaugccGCGGaAUGCuCGUCu -3' miRNA: 3'- -GGCU-CUUGGCCU------UGCUgUACG-GCAGc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 56861 | 0.66 | 0.869379 |
Target: 5'- cUCGGGAcCuCGGuAGCGGCugcgGUCGUCGu -3' miRNA: 3'- -GGCUCUuG-GCC-UUGCUGua--CGGCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 51875 | 0.66 | 0.869379 |
Target: 5'- uUGAGGuuGCgGGcuGugGACAUGCCGagGg -3' miRNA: 3'- gGCUCU--UGgCC--UugCUGUACGGCagC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 44758 | 0.66 | 0.856492 |
Target: 5'- gCCGAGAuccaaaccuacgacCCGGcGGCGGCggGCCG-CGu -3' miRNA: 3'- -GGCUCUu-------------GGCC-UUGCUGuaCGGCaGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 11373 | 0.66 | 0.853178 |
Target: 5'- gCCGAGAuCgCGGAACuugGUGCCGUa- -3' miRNA: 3'- -GGCUCUuG-GCCUUGcugUACGGCAgc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 51167 | 0.66 | 0.844733 |
Target: 5'- gCG-GAACUGGAucCGGCGcGCCGcCGa -3' miRNA: 3'- gGCuCUUGGCCUu-GCUGUaCGGCaGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 15947 | 0.66 | 0.844733 |
Target: 5'- aCGAGuuCUGGGACGACAUcgagaauucggcGCCGa-- -3' miRNA: 3'- gGCUCuuGGCCUUGCUGUA------------CGGCagc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 13786 | 0.66 | 0.844733 |
Target: 5'- aCCGGcGaAACCGGAuGCGACcuUGCCGa-- -3' miRNA: 3'- -GGCU-C-UUGGCCU-UGCUGu-ACGGCagc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 35618 | 0.66 | 0.836071 |
Target: 5'- aCGaAGAGgCGGcAACGGCAuaUGCCaUCGg -3' miRNA: 3'- gGC-UCUUgGCC-UUGCUGU--ACGGcAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 3376 | 0.67 | 0.830773 |
Target: 5'- cCCG-GAuguUCGGuugugguacgacgccGACGACGUGCCGUUc -3' miRNA: 3'- -GGCuCUu--GGCC---------------UUGCUGUACGGCAGc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 49443 | 0.67 | 0.827199 |
Target: 5'- uCCGGGGcagguGCaGGGGC-ACcgGCCGUCGu -3' miRNA: 3'- -GGCUCU-----UGgCCUUGcUGuaCGGCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 37006 | 0.67 | 0.826301 |
Target: 5'- uCCGGGAuaccGCCGGGGCGAUGgcgGauucucgaccacaCCGUCu -3' miRNA: 3'- -GGCUCU----UGGCCUUGCUGUa--C-------------GGCAGc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 38661 | 0.67 | 0.818127 |
Target: 5'- gCGAGGGCU-GAACGugAUGCgcucgcuggccgCGUCGa -3' miRNA: 3'- gGCUCUUGGcCUUGCugUACG------------GCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 4214 | 0.67 | 0.818127 |
Target: 5'- cCCGAGAucggGCCGG--UGACGUGgCCGg-- -3' miRNA: 3'- -GGCUCU----UGGCCuuGCUGUAC-GGCagc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 23355 | 0.67 | 0.818127 |
Target: 5'- gCCGGGGACCGG----GC-UGCgGUCGa -3' miRNA: 3'- -GGCUCUUGGCCuugcUGuACGgCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 6736 | 0.67 | 0.808863 |
Target: 5'- gUCGAcGGCCcGGGCGGCcgcGUGCUGUCGc -3' miRNA: 3'- -GGCUcUUGGcCUUGCUG---UACGGCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 42895 | 0.67 | 0.808863 |
Target: 5'- cCCGAGGuGCaCGGGcugaaGCgGACGguacGCCGUCGg -3' miRNA: 3'- -GGCUCU-UG-GCCU-----UG-CUGUa---CGGCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 51325 | 0.67 | 0.799419 |
Target: 5'- gCCGAGGGCCuucuGCGACGgcuUGCCGa-- -3' miRNA: 3'- -GGCUCUUGGccu-UGCUGU---ACGGCagc -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 39649 | 0.67 | 0.799419 |
Target: 5'- cCCGAGGGCCGcuacguCGAgAUGCuuggaaCGUCGg -3' miRNA: 3'- -GGCUCUUGGCcuu---GCUgUACG------GCAGC- -5' |
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18759 | 3' | -53.5 | NC_004683.1 | + | 53101 | 0.67 | 0.799419 |
Target: 5'- cCCGAGccaucGGCCGGGGCGuuc-GUCGUUGg -3' miRNA: 3'- -GGCUC-----UUGGCCUUGCuguaCGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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