Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18765 | 3' | -64.2 | NC_004683.1 | + | 12870 | 0.66 | 0.320974 |
Target: 5'- aUCCGccagCGGCgUauccCGCCGGGUAGCcguagCCc -3' miRNA: 3'- -AGGCa---GCCGgA----GCGGCCCGUCGa----GGu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 28544 | 0.66 | 0.320974 |
Target: 5'- --aGUCGGCCguugCGCCGgGGCAauccccCUCCc -3' miRNA: 3'- aggCAGCCGGa---GCGGC-CCGUc-----GAGGu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 52217 | 0.66 | 0.313629 |
Target: 5'- gCCGUCGucggucggguccGCCUCggcgacccggGCCGGcGCGGCaCCAa -3' miRNA: 3'- aGGCAGC------------CGGAG----------CGGCC-CGUCGaGGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 4449 | 0.66 | 0.313629 |
Target: 5'- gUCGUCGcuggcGUCUCGCUGcGCGGCUUCGu -3' miRNA: 3'- aGGCAGC-----CGGAGCGGCcCGUCGAGGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 31893 | 0.66 | 0.313629 |
Target: 5'- aUCGUCGGCuCggGCCGGuGCAGC-Ca- -3' miRNA: 3'- aGGCAGCCG-GagCGGCC-CGUCGaGgu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 25874 | 0.66 | 0.313629 |
Target: 5'- gCUG-CGGCUcggcgucgaguUCGCCGGGCAGaCgcuggCCGc -3' miRNA: 3'- aGGCaGCCGG-----------AGCGGCCCGUC-Ga----GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 8368 | 0.66 | 0.31145 |
Target: 5'- gCCGucuccugacggugaUCGGCCcCGCgCGGGCcGCgaUCCAg -3' miRNA: 3'- aGGC--------------AGCCGGaGCG-GCCCGuCG--AGGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 29496 | 0.66 | 0.299322 |
Target: 5'- aCCGUCGGaCCUCaccaagggGCCGGGaacgugGGuUUCCAa -3' miRNA: 3'- aGGCAGCC-GGAG--------CGGCCCg-----UC-GAGGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 21938 | 0.66 | 0.299322 |
Target: 5'- aUCCGccCGGCggUGgCGGGCGGCcgCCGa -3' miRNA: 3'- -AGGCa-GCCGgaGCgGCCCGUCGa-GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 11787 | 0.66 | 0.299322 |
Target: 5'- aCCGUCGGCg-UGCCGcuGCAgcGCUCCc -3' miRNA: 3'- aGGCAGCCGgaGCGGCc-CGU--CGAGGu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 22025 | 0.66 | 0.299322 |
Target: 5'- cUCGgCGGCCgcaccgccacCGCCGGGCGGauugCCGg -3' miRNA: 3'- aGGCaGCCGGa---------GCGGCCCGUCga--GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 46675 | 0.66 | 0.299322 |
Target: 5'- gCCGUcgCGGcCCUCGaCCGGcaccaGCAGCggcagCCAg -3' miRNA: 3'- aGGCA--GCC-GGAGC-GGCC-----CGUCGa----GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 41394 | 0.66 | 0.278818 |
Target: 5'- gCCGUCaGCCUgGgCGGGUGGCgcggugaggCCGg -3' miRNA: 3'- aGGCAGcCGGAgCgGCCCGUCGa--------GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 40883 | 0.66 | 0.278818 |
Target: 5'- cUCGcacUCGGCCUCGCCGucGGCgAGCgcgUCGg -3' miRNA: 3'- aGGC---AGCCGGAGCGGC--CCG-UCGa--GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 340 | 0.66 | 0.278818 |
Target: 5'- aCCGUCGcgaccaGCCcCGCCgcGGGCAGCagCGg -3' miRNA: 3'- aGGCAGC------CGGaGCGG--CCCGUCGagGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 13661 | 0.67 | 0.26578 |
Target: 5'- gCCGUCGGgCUCGCCacuGGGCAacaUCg- -3' miRNA: 3'- aGGCAGCCgGAGCGG---CCCGUcg-AGgu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 28781 | 0.67 | 0.263867 |
Target: 5'- cUCGUCGacacgggaucugaaGCCggCGCUGGGCAGgcCUCCGc -3' miRNA: 3'- aGGCAGC--------------CGGa-GCGGCCCGUC--GAGGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 8976 | 0.67 | 0.259448 |
Target: 5'- gUUGUUGGCCagccgcugaaUCGCCGGGUacaGGUUCUg -3' miRNA: 3'- aGGCAGCCGG----------AGCGGCCCG---UCGAGGu -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 40959 | 0.67 | 0.25324 |
Target: 5'- cCCGUCGGCCaCGCCGaccuGGCcGCgcgcaucgCCGc -3' miRNA: 3'- aGGCAGCCGGaGCGGC----CCGuCGa-------GGU- -5' |
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18765 | 3' | -64.2 | NC_004683.1 | + | 2503 | 0.67 | 0.25324 |
Target: 5'- --aGUUGGUguuCUCGUCGGuGCGGUUCCGg -3' miRNA: 3'- aggCAGCCG---GAGCGGCC-CGUCGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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