Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18769 | 5' | -58.5 | NC_004683.1 | + | 30605 | 0.66 | 0.583298 |
Target: 5'- cGCGCCCuCGGAUaccaaacUGCCUGac-GCGCa -3' miRNA: 3'- -CGUGGG-GCCUAca-----GCGGACcuaCGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 13548 | 0.66 | 0.562179 |
Target: 5'- aCGCCCUGGAaGcCGCCgccacccUGCGCg -3' miRNA: 3'- cGUGGGGCCUaCaGCGGaccu---ACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 20368 | 0.66 | 0.562179 |
Target: 5'- cCGCCCCGGccGg-GUUUGGAUGCu- -3' miRNA: 3'- cGUGGGGCCuaCagCGGACCUACGcg -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 21647 | 0.66 | 0.562179 |
Target: 5'- aCACCCUGGAgcgcggCGUCcauaucccgUGGAcUGCGCg -3' miRNA: 3'- cGUGGGGCCUaca---GCGG---------ACCU-ACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 32866 | 0.66 | 0.551695 |
Target: 5'- aGCuCCCaCGcGAUcgcguagcgGUCGCCUGcGAuaUGCGCc -3' miRNA: 3'- -CGuGGG-GC-CUA---------CAGCGGAC-CU--ACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 7711 | 0.67 | 0.520631 |
Target: 5'- -gGCCgUGGcGUGUUcggcccgaaaGCCUGGuAUGCGCa -3' miRNA: 3'- cgUGGgGCC-UACAG----------CGGACC-UACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 52064 | 0.67 | 0.510426 |
Target: 5'- cGCGCCCUGGcUGaUCGUCgacgcgGGAgGCGa -3' miRNA: 3'- -CGUGGGGCCuAC-AGCGGa-----CCUaCGCg -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 12794 | 0.67 | 0.510426 |
Target: 5'- cGCGCCCCGcg---CGCCgaGGAUGC-Cg -3' miRNA: 3'- -CGUGGGGCcuacaGCGGa-CCUACGcG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 9215 | 0.67 | 0.490275 |
Target: 5'- aCACCUucauCGGcgGUccCGCCUGGGacGCGCu -3' miRNA: 3'- cGUGGG----GCCuaCA--GCGGACCUa-CGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 39612 | 0.67 | 0.490275 |
Target: 5'- gGCGCCCguuccgGGGUGggCGCCgccgGGGUG-GCg -3' miRNA: 3'- -CGUGGGg-----CCUACa-GCGGa---CCUACgCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 13147 | 0.67 | 0.490275 |
Target: 5'- cGUGCCaCCGGcagcGUCGCCUGcAUGUGg -3' miRNA: 3'- -CGUGG-GGCCua--CAGCGGACcUACGCg -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 15165 | 0.67 | 0.480338 |
Target: 5'- cGCACCgaugCUGGG-GUgGCCUGGGUGaggaCGCc -3' miRNA: 3'- -CGUGG----GGCCUaCAgCGGACCUAC----GCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 36929 | 0.68 | 0.470501 |
Target: 5'- uCGCCCCGGcgGUauCCcGGAggucagcuUGCGCu -3' miRNA: 3'- cGUGGGGCCuaCAgcGGaCCU--------ACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 7985 | 0.68 | 0.464648 |
Target: 5'- aCGCCCCcgacgaagaGGAUuucGUCGUCUGcuggaugcagccgguGAUGCGCa -3' miRNA: 3'- cGUGGGG---------CCUA---CAGCGGAC---------------CUACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 6704 | 0.68 | 0.460767 |
Target: 5'- -aGCCUCGGuguUGUCGgcgaaCUGGccGCGCa -3' miRNA: 3'- cgUGGGGCCu--ACAGCg----GACCuaCGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 7380 | 0.68 | 0.451141 |
Target: 5'- aGCA-CCCGGAggcgcUCGaCCUGGAUucggcggccggGCGCg -3' miRNA: 3'- -CGUgGGGCCUac---AGC-GGACCUA-----------CGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 46064 | 0.68 | 0.451141 |
Target: 5'- cGUGCCCCGGu--UCGCCgag--GCGCa -3' miRNA: 3'- -CGUGGGGCCuacAGCGGaccuaCGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 46011 | 0.68 | 0.431293 |
Target: 5'- uGCACUCCGGGc-UCGCCcagagaaUGucGGUGCGCg -3' miRNA: 3'- -CGUGGGGCCUacAGCGG-------AC--CUACGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 9340 | 0.69 | 0.417433 |
Target: 5'- uGCGCCCCGcGAgggucuuacgagccGUCGCC-GGccaGCGCg -3' miRNA: 3'- -CGUGGGGC-CUa-------------CAGCGGaCCua-CGCG- -5' |
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18769 | 5' | -58.5 | NC_004683.1 | + | 21572 | 0.69 | 0.395832 |
Target: 5'- -uGCCCCGGcuguggugaAUGUCGCCgccagcGGG-GCGUg -3' miRNA: 3'- cgUGGGGCC---------UACAGCGGa-----CCUaCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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