Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19269 | 3' | -50.1 | NC_004685.1 | + | 68267 | 0.66 | 0.97016 |
Target: 5'- aCGCgCAUGAgGAgG--UGUUCGGAAGCc -3' miRNA: 3'- -GUG-GUACUgCUgUggACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 57090 | 0.66 | 0.97016 |
Target: 5'- gCACCucGGCGGCGC--GUUCGGAcACg -3' miRNA: 3'- -GUGGuaCUGCUGUGgaCAAGCCUuUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 51224 | 0.66 | 0.963262 |
Target: 5'- cCACCAUGcCGAgACUg--UCGGAcGCc -3' miRNA: 3'- -GUGGUACuGCUgUGGacaAGCCUuUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 65323 | 0.66 | 0.959426 |
Target: 5'- gGCCAuuUGacGCGAUACCcg-UCGGGGACc -3' miRNA: 3'- gUGGU--AC--UGCUGUGGacaAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 19697 | 0.67 | 0.950946 |
Target: 5'- gGCCGUGGCGG-GCCgaggcGUUCGGccACa -3' miRNA: 3'- gUGGUACUGCUgUGGa----CAAGCCuuUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 68548 | 0.67 | 0.944842 |
Target: 5'- gGCCAguccGAUgcgGGCGCCccgaugcgucggggUGUUCGGAAACc -3' miRNA: 3'- gUGGUa---CUG---CUGUGG--------------ACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 47550 | 0.67 | 0.941358 |
Target: 5'- uGCCAggUGGCgGGCAgggUGUUCGGAAACc -3' miRNA: 3'- gUGGU--ACUG-CUGUgg-ACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 33181 | 0.67 | 0.936139 |
Target: 5'- gGCCGgugGugGGCGCCgGUgCGGuGGACg -3' miRNA: 3'- gUGGUa--CugCUGUGGaCAaGCC-UUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 66555 | 0.67 | 0.930633 |
Target: 5'- gGCCAagucGGCGGC-CCUG--CGGAAGCg -3' miRNA: 3'- gUGGUa---CUGCUGuGGACaaGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 2421 | 0.68 | 0.924244 |
Target: 5'- uGCCA-GACGACgcggccgccggugGCCUGUUCGacGGCg -3' miRNA: 3'- gUGGUaCUGCUG-------------UGGACAAGCcuUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 17205 | 0.68 | 0.905737 |
Target: 5'- uCGCCAgaaACGACugagUgUGUUCGGAAACc -3' miRNA: 3'- -GUGGUac-UGCUGu---GgACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 4505 | 0.68 | 0.905737 |
Target: 5'- cCGCagGUGAUucugGCCUGUUCGGAAACc -3' miRNA: 3'- -GUGg-UACUGcug-UGGACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 8392 | 0.68 | 0.905737 |
Target: 5'- gUACCccGACGGCaACCUGUgccugCGGcuGCc -3' miRNA: 3'- -GUGGuaCUGCUG-UGGACAa----GCCuuUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 466 | 0.68 | 0.905737 |
Target: 5'- -cCCGUGGcCGcCACCaUG-UCGGAGACg -3' miRNA: 3'- guGGUACU-GCuGUGG-ACaAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 43393 | 0.68 | 0.898801 |
Target: 5'- gCACCAUGAUGACcucGCCguUGUUCaGGGu-- -3' miRNA: 3'- -GUGGUACUGCUG---UGG--ACAAG-CCUuug -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 43985 | 0.68 | 0.898801 |
Target: 5'- cCGCCGUGGgagcgUGACAgCCUGga-GGAAACc -3' miRNA: 3'- -GUGGUACU-----GCUGU-GGACaagCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 14956 | 0.69 | 0.884099 |
Target: 5'- cCGCCGUGGCGuGCGCucugcgCUGUUCagguGGggGCu -3' miRNA: 3'- -GUGGUACUGC-UGUG------GACAAG----CCuuUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 2595 | 0.69 | 0.884099 |
Target: 5'- cCGCCAgGGCGGCauaccgcucgGCCUGcUCGGcAGCg -3' miRNA: 3'- -GUGGUaCUGCUG----------UGGACaAGCCuUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 19378 | 0.69 | 0.884099 |
Target: 5'- aGCCcgcUGAUGAacugaCUGUUCGGAAACc -3' miRNA: 3'- gUGGu--ACUGCUgug--GACAAGCCUUUG- -5' |
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19269 | 3' | -50.1 | NC_004685.1 | + | 58737 | 0.69 | 0.860047 |
Target: 5'- uCAUCAUGACGaACGCCUGccCGGc-GCg -3' miRNA: 3'- -GUGGUACUGC-UGUGGACaaGCCuuUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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