Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19281 | 5' | -56.7 | NC_004685.1 | + | 10536 | 0.66 | 0.708494 |
Target: 5'- -cGCcGCGGCCGGGUG-GCuucgacaaCGUGGg -3' miRNA: 3'- acUGaCGUCGGUCCACaCGua------GCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 14948 | 0.66 | 0.708494 |
Target: 5'- aUGGCggUGCGGCguGaGUucGUGCGUCGCu- -3' miRNA: 3'- -ACUG--ACGUCGguC-CA--CACGUAGCGcc -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 54013 | 0.66 | 0.708494 |
Target: 5'- -uGCUGcCGGUggaugugccaugCAGGUGUGCGgggaugcCGCGGg -3' miRNA: 3'- acUGAC-GUCG------------GUCCACACGUa------GCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 24978 | 0.66 | 0.687299 |
Target: 5'- gUGAUgaacaGCGGCCGGGUGUcCGgccCGgGGg -3' miRNA: 3'- -ACUGa----CGUCGGUCCACAcGUa--GCgCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 5429 | 0.66 | 0.676621 |
Target: 5'- gGACaGCcuGGCCAgGGUGgaccUGUAUCuGCGGg -3' miRNA: 3'- aCUGaCG--UCGGU-CCAC----ACGUAG-CGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 38842 | 0.66 | 0.676621 |
Target: 5'- cGGCUGCcgcGCCGGucgGUGCc-CGCGGu -3' miRNA: 3'- aCUGACGu--CGGUCca-CACGuaGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 19909 | 0.66 | 0.67555 |
Target: 5'- gGGUUGCuGCCGaugacgcGG-GUGUAUCGCGGc -3' miRNA: 3'- aCUGACGuCGGU-------CCaCACGUAGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 8667 | 0.66 | 0.665901 |
Target: 5'- cUGAgUGCGGCaCGacGaUGUGCAUCGcCGGc -3' miRNA: 3'- -ACUgACGUCG-GU--CcACACGUAGC-GCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 50666 | 0.67 | 0.644382 |
Target: 5'- cGACaggGCAGCCAGcGUGcGCcacaccuUCGCGu -3' miRNA: 3'- aCUGa--CGUCGGUC-CACaCGu------AGCGCc -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 7740 | 0.67 | 0.644382 |
Target: 5'- aGGCccGCAGCCAGGacGUGCAcgacCGCa- -3' miRNA: 3'- aCUGa-CGUCGGUCCa-CACGUa---GCGcc -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 41850 | 0.68 | 0.5692 |
Target: 5'- gUGcACgGCGGCCGGGUGUuCcgCGCcGGu -3' miRNA: 3'- -AC-UGaCGUCGGUCCACAcGuaGCG-CC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 6555 | 0.68 | 0.558593 |
Target: 5'- cGACaaGCAG-CAGGUGcGCAUCGUGc -3' miRNA: 3'- aCUGa-CGUCgGUCCACaCGUAGCGCc -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 64181 | 0.68 | 0.558593 |
Target: 5'- cGACUuggcGguGCCGcGGUGUcGaguaGUCGCGGa -3' miRNA: 3'- aCUGA----CguCGGU-CCACA-Cg---UAGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 63247 | 0.68 | 0.558593 |
Target: 5'- gGGCUGUucgGGCCAGGUGaUGCccuUgGCGc -3' miRNA: 3'- aCUGACG---UCGGUCCAC-ACGu--AgCGCc -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 7152 | 0.69 | 0.527136 |
Target: 5'- cGugUuCGGCgGGGUG-GcCAUCGCGGu -3' miRNA: 3'- aCugAcGUCGgUCCACaC-GUAGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 6021 | 0.69 | 0.516795 |
Target: 5'- cGAUgccGCGGCgGGGUGaUGUGUUGUGGc -3' miRNA: 3'- aCUGa--CGUCGgUCCAC-ACGUAGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 66203 | 0.69 | 0.516795 |
Target: 5'- cGugUGCGGCCacgacgccaacaAGGgcugcccucaGUGCAUCcGCGGu -3' miRNA: 3'- aCugACGUCGG------------UCCa---------CACGUAG-CGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 25561 | 0.69 | 0.516795 |
Target: 5'- gGACUGCAGCgCGGuG-GUGCGcUUGuCGGa -3' miRNA: 3'- aCUGACGUCG-GUC-CaCACGU-AGC-GCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 57555 | 0.69 | 0.505514 |
Target: 5'- -aGCUGCGcGCCAGGccgaucaucucgaUG-GCGUUGCGGu -3' miRNA: 3'- acUGACGU-CGGUCC-------------ACaCGUAGCGCC- -5' |
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19281 | 5' | -56.7 | NC_004685.1 | + | 31240 | 0.69 | 0.486287 |
Target: 5'- cGGCUcgGGCCuGGUcgGCAUCGCGGc -3' miRNA: 3'- aCUGAcgUCGGuCCAcaCGUAGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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