Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19558 | 3' | -58.8 | NC_004686.1 | + | 32727 | 0.66 | 0.568959 |
Target: 5'- cGUUCGAGCGcuggaaGAUGCUuccuucucccuuugcUCGGgGUGGGCg -3' miRNA: 3'- -CGGGCUUGC------CUGCGA---------------AGCCgCGUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 21472 | 0.66 | 0.564768 |
Target: 5'- aGUCUGGu--GAUGUUUCGGCGUugucgGGGCg -3' miRNA: 3'- -CGGGCUugcCUGCGAAGCCGCG-----UCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 52874 | 0.66 | 0.564768 |
Target: 5'- gGUCgGAAugaaacguCGGGCGCcaggaucaugCGGCGCAuGGCu -3' miRNA: 3'- -CGGgCUU--------GCCUGCGaa--------GCCGCGU-CCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 39165 | 0.66 | 0.554326 |
Target: 5'- uGCUCGAaugagugccgACGGGCGCagaGGUG-GGGCg -3' miRNA: 3'- -CGGGCU----------UGCCUGCGaagCCGCgUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 791 | 0.66 | 0.554326 |
Target: 5'- uGCCCGGuAUGGGgauugUGCUcgaUCGGUgGCAGGa -3' miRNA: 3'- -CGGGCU-UGCCU-----GCGA---AGCCG-CGUCCg -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 15438 | 0.66 | 0.553285 |
Target: 5'- aCCCGGAagaagcauuucugUGGAUGCUggUGGCGU-GGCc -3' miRNA: 3'- cGGGCUU-------------GCCUGCGAa-GCCGCGuCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 19009 | 0.66 | 0.543941 |
Target: 5'- aGCCgaagUGGGCGaACGCguuugaccugUUCGGUGUGGGCg -3' miRNA: 3'- -CGG----GCUUGCcUGCG----------AAGCCGCGUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 10326 | 0.66 | 0.543941 |
Target: 5'- aGCCUGAugGG-CGCUgacccaguuauUCagguuguccagGGCGguGGUg -3' miRNA: 3'- -CGGGCUugCCuGCGA-----------AG-----------CCGCguCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 38760 | 0.66 | 0.533621 |
Target: 5'- aCCCcc-UGGuuCGCUaggUGGCGCGGGCa -3' miRNA: 3'- cGGGcuuGCCu-GCGAa--GCCGCGUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 27460 | 0.67 | 0.523371 |
Target: 5'- cGCCgCGccaaGGACGCUaUCuGuGCGUGGGCa -3' miRNA: 3'- -CGG-GCuug-CCUGCGA-AG-C-CGCGUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 51508 | 0.67 | 0.523371 |
Target: 5'- uGCUCGuguGgGGGCGUggaucgCGGCGUGGGa -3' miRNA: 3'- -CGGGCu--UgCCUGCGaa----GCCGCGUCCg -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 39762 | 0.67 | 0.513198 |
Target: 5'- cGCCUccuacACGGACGCcgCGGCGacggugGGGUg -3' miRNA: 3'- -CGGGcu---UGCCUGCGaaGCCGCg-----UCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 55061 | 0.67 | 0.513198 |
Target: 5'- gGUuuGuGCGGugGCg-CGGuUGUAGGCg -3' miRNA: 3'- -CGggCuUGCCugCGaaGCC-GCGUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 22226 | 0.67 | 0.510161 |
Target: 5'- gGCCaGAgcagaaccaacggcGCGGGCGCUggCGGUaaGGGCg -3' miRNA: 3'- -CGGgCU--------------UGCCUGCGAa-GCCGcgUCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 8988 | 0.67 | 0.503106 |
Target: 5'- gGUCCauccGCGGGCGaggaggUCGGCGCGgauGGCc -3' miRNA: 3'- -CGGGcu--UGCCUGCga----AGCCGCGU---CCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 45810 | 0.67 | 0.493103 |
Target: 5'- gGCCuUGGguGCGGGaGCaUUCGGCGCAaGCa -3' miRNA: 3'- -CGG-GCU--UGCCUgCG-AAGCCGCGUcCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 43053 | 0.67 | 0.483191 |
Target: 5'- aGCCCGu-UGGACGCcacgUUGGUGUcuucgcGGCg -3' miRNA: 3'- -CGGGCuuGCCUGCGa---AGCCGCGu-----CCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 47503 | 0.67 | 0.473376 |
Target: 5'- gGUgCGAACGGGaGaCUUCGGCggaGCGGGa -3' miRNA: 3'- -CGgGCUUGCCUgC-GAAGCCG---CGUCCg -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 22665 | 0.68 | 0.444555 |
Target: 5'- gGCCCGcaucaucgaaccGGCGGcaGCGUUcaUCGGUGUaucGGGCg -3' miRNA: 3'- -CGGGC------------UUGCC--UGCGA--AGCCGCG---UCCG- -5' |
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19558 | 3' | -58.8 | NC_004686.1 | + | 3382 | 0.68 | 0.43891 |
Target: 5'- aUgCGAagugGCGGGaucCGCgguaucugucgaagUCGGCGCAGGCu -3' miRNA: 3'- cGgGCU----UGCCU---GCGa-------------AGCCGCGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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