Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19560 | 3' | -49.8 | NC_004686.1 | + | 18579 | 0.66 | 0.951154 |
Target: 5'- aUCGgcuCCGAcuuggcGGCACGAuagGUGAugGCCa-- -3' miRNA: 3'- -AGC---GGUU------UCGUGCU---UACUugCGGaag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 39843 | 0.66 | 0.951154 |
Target: 5'- cCGCCGGcAGUgcgcugaaugGCGAGUGAuCGCCcgCa -3' miRNA: 3'- aGCGGUU-UCG----------UGCUUACUuGCGGaaG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 48418 | 0.66 | 0.946394 |
Target: 5'- cCGCCGugaguucgacGGGCGCGA--GGACaaguacugGCCUUCg -3' miRNA: 3'- aGCGGU----------UUCGUGCUuaCUUG--------CGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 32352 | 0.66 | 0.935983 |
Target: 5'- aCGCCcgguucagcAGCACGu----GCGCCUUCu -3' miRNA: 3'- aGCGGuu-------UCGUGCuuacuUGCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 26323 | 0.66 | 0.935431 |
Target: 5'- aCGCCAAugcgcacaugaucGGCAuCGAAU--GCGCCUg- -3' miRNA: 3'- aGCGGUU-------------UCGU-GCUUAcuUGCGGAag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 3258 | 0.66 | 0.929746 |
Target: 5'- aUCGCUgaggcugAAGGCGCGGuggAUGAuuucACGCCggCg -3' miRNA: 3'- -AGCGG-------UUUCGUGCU---UACU----UGCGGaaG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 17802 | 0.67 | 0.923757 |
Target: 5'- gCGCCAc--CGCGAucacaucuucgcuGUGGAuCGCCUUCu -3' miRNA: 3'- aGCGGUuucGUGCU-------------UACUU-GCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 35354 | 0.67 | 0.91811 |
Target: 5'- aCGCCGAcGC-CGGAaccGAcaacuccaACGCCUUCg -3' miRNA: 3'- aGCGGUUuCGuGCUUa--CU--------UGCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 31363 | 0.67 | 0.91811 |
Target: 5'- aCGCCGaauucGAGCACGuguggGAACGCa--- -3' miRNA: 3'- aGCGGU-----UUCGUGCuua--CUUGCGgaag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 27233 | 0.68 | 0.890083 |
Target: 5'- gUCGCCGAccGGUuuCcAGUGAcacACGCCUUCc -3' miRNA: 3'- -AGCGGUU--UCGu-GcUUACU---UGCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 4583 | 0.68 | 0.874331 |
Target: 5'- cCGCCAAAGCG-GAAgcguuGCGgCUUCg -3' miRNA: 3'- aGCGGUUUCGUgCUUacu--UGCgGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 4268 | 0.68 | 0.874331 |
Target: 5'- aUUGCCGcacgcGGGCACGAucuuccggcGUGGACGgCUUa -3' miRNA: 3'- -AGCGGU-----UUCGUGCU---------UACUUGCgGAAg -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 38333 | 0.68 | 0.857484 |
Target: 5'- aUCGUCAGacAGgGCGAAUGAAUGUUggCg -3' miRNA: 3'- -AGCGGUU--UCgUGCUUACUUGCGGaaG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 29393 | 0.69 | 0.846877 |
Target: 5'- -gGCCGAuggugauguucuGCAUGAggGAAUGCCUUUc -3' miRNA: 3'- agCGGUUu-----------CGUGCUuaCUUGCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 21249 | 0.69 | 0.829364 |
Target: 5'- gUGCCAGcccccgcGGCGCGGAacAACGCCUg- -3' miRNA: 3'- aGCGGUU-------UCGUGCUUacUUGCGGAag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 22550 | 0.69 | 0.820778 |
Target: 5'- cCGCCGGGGCGgGGcugAUGAugGUCUc- -3' miRNA: 3'- aGCGGUUUCGUgCU---UACUugCGGAag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 47951 | 0.7 | 0.780612 |
Target: 5'- cUCGCC--GGCGCGAcgGAA-GCCUc- -3' miRNA: 3'- -AGCGGuuUCGUGCUuaCUUgCGGAag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 46755 | 0.7 | 0.780612 |
Target: 5'- -gGCCAAGGCugGggUGAuucaccCGCUg-- -3' miRNA: 3'- agCGGUUUCGugCuuACUu-----GCGGaag -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 33880 | 0.7 | 0.75947 |
Target: 5'- cCGCgAGGGCGaGGAUGAcuuCGCCUUUg -3' miRNA: 3'- aGCGgUUUCGUgCUUACUu--GCGGAAG- -5' |
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19560 | 3' | -49.8 | NC_004686.1 | + | 36376 | 0.7 | 0.75947 |
Target: 5'- -aGCUcgGGCACGAgaGUGcGCGCCUg- -3' miRNA: 3'- agCGGuuUCGUGCU--UACuUGCGGAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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