Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19560 | 5' | -51.2 | NC_004686.1 | + | 51097 | 0.66 | 0.886354 |
Target: 5'- -----cUCGUCCGCCGCGaggcuguaCCUggaCAGCc -3' miRNA: 3'- cacuuuAGUAGGUGGCGC--------GGAa--GUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 36833 | 0.66 | 0.886354 |
Target: 5'- --cGGGUCGUCaccacgACCGUGCCgaUCGGCg -3' miRNA: 3'- cacUUUAGUAGg-----UGGCGCGGa-AGUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 39269 | 0.66 | 0.901074 |
Target: 5'- -cGGccGGUCAUgcgccCCACCucuGCGCCcgUCGGCa -3' miRNA: 3'- caCU--UUAGUA-----GGUGG---CGCGGa-AGUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 24413 | 0.66 | 0.901074 |
Target: 5'- -----cUUGUCCGCCGCuGCCggUGGCg -3' miRNA: 3'- cacuuuAGUAGGUGGCG-CGGaaGUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 42085 | 0.66 | 0.908005 |
Target: 5'- cUGGGAuugccccguacUCGUCgGCgGCaCCUUCAGCc -3' miRNA: 3'- cACUUU-----------AGUAGgUGgCGcGGAAGUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 12103 | 0.66 | 0.908005 |
Target: 5'- cUGcug-CggCUACCGCGgCUUCGGCu -3' miRNA: 3'- cACuuuaGuaGGUGGCGCgGAAGUCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 25335 | 0.66 | 0.914645 |
Target: 5'- -cGAucUCAUCgGCCaGUGCUUUCgcAGCa -3' miRNA: 3'- caCUuuAGUAGgUGG-CGCGGAAG--UCG- -5' |
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19560 | 5' | -51.2 | NC_004686.1 | + | 14594 | 0.66 | 0.914645 |
Target: 5'- -gGAGGUUGg-CGCCGCGCCa-CGGCu -3' miRNA: 3'- caCUUUAGUagGUGGCGCGGaaGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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