Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19560 | 5' | -51.2 | NC_004686.1 | + | 43752 | 0.71 | 0.64495 |
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Target: 5'- ---------gCCGCCGCGCCaUCGGCg -3' miRNA: 3'- cacuuuaguaGGUGGCGCGGaAGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 47604 | 0.67 | 0.862212 |
|
Target: 5'- aUGAAGUCgAUUgAUCGUGCCUgacCGGUa -3' miRNA: 3'- cACUUUAG-UAGgUGGCGCGGAa--GUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 47897 | 0.68 | 0.797363 |
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Target: 5'- aUGAcAUCcgCCcCCGCGCCaa-AGCg -3' miRNA: 3'- cACUuUAGuaGGuGGCGCGGaagUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 48915 | 0.7 | 0.701385 |
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Target: 5'- -cGGAAUCuggCCGCCGC-CCUUCucGCc -3' miRNA: 3'- caCUUUAGua-GGUGGCGcGGAAGu-CG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 49975 | 0.68 | 0.796362 |
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Target: 5'- gGUGAucucgauGAUCGuUUCGCCGCGgCCUUCGucGCc -3' miRNA: 3'- -CACU-------UUAGU-AGGUGGCGC-GGAAGU--CG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 51097 | 0.66 | 0.886354 |
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Target: 5'- -----cUCGUCCGCCGCGaggcuguaCCUggaCAGCc -3' miRNA: 3'- cacuuuAGUAGGUGGCGC--------GGAa--GUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 54799 | 0.67 | 0.869706 |
|
Target: 5'- aGUGGAG-CAUCCGCCucucaugacuacgGuCGCCUUCAu- -3' miRNA: 3'- -CACUUUaGUAGGUGG-------------C-GCGGAAGUcg -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 55935 | 0.76 | 0.389248 |
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Target: 5'- ------cCAUCgACCGCGCCaUCAGCa -3' miRNA: 3'- cacuuuaGUAGgUGGCGCGGaAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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