Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19560 | 5' | -51.2 | NC_004686.1 | + | 30364 | 0.79 | 0.252862 |
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Target: 5'- -cGAGAUCcgguUCCAUCGCGCCaUCGGUg -3' miRNA: 3'- caCUUUAGu---AGGUGGCGCGGaAGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 33957 | 0.72 | 0.588186 |
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Target: 5'- -cGAAGUCAUCCucgcccucgcgGCCG-GCCUUC-GCg -3' miRNA: 3'- caCUUUAGUAGG-----------UGGCgCGGAAGuCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 10145 | 0.72 | 0.610843 |
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Target: 5'- aUGuuGUCAUgCACCGCGCgCUggaaguccgCGGCa -3' miRNA: 3'- cACuuUAGUAgGUGGCGCG-GAa--------GUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 31301 | 0.7 | 0.712505 |
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Target: 5'- -aGAAcggCAaCCugCGCGCCcgCAGCg -3' miRNA: 3'- caCUUua-GUaGGugGCGCGGaaGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 35194 | 0.68 | 0.796362 |
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Target: 5'- --uGAGUCAUCCACCGCuugaacgGCUUcacccucggagaUCGGCu -3' miRNA: 3'- cacUUUAGUAGGUGGCG-------CGGA------------AGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 49975 | 0.68 | 0.796362 |
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Target: 5'- gGUGAucucgauGAUCGuUUCGCCGCGgCCUUCGucGCc -3' miRNA: 3'- -CACU-------UUAGU-AGGUGGCGC-GGAAGU--CG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 47897 | 0.68 | 0.797363 |
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Target: 5'- aUGAcAUCcgCCcCCGCGCCaa-AGCg -3' miRNA: 3'- cACUuUAGuaGGuGGCGCGGaagUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 14594 | 0.66 | 0.914645 |
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Target: 5'- -gGAGGUUGg-CGCCGCGCCa-CGGCu -3' miRNA: 3'- caCUUUAGUagGUGGCGCGGaaGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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