Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19560 | 5' | -51.2 | NC_004686.1 | + | 48915 | 0.7 | 0.701385 |
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Target: 5'- -cGGAAUCuggCCGCCGC-CCUUCucGCc -3' miRNA: 3'- caCUUUAGua-GGUGGCGcGGAAGu-CG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 30530 | 0.69 | 0.766585 |
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Target: 5'- gGUGGAGUgAUCCGggUCGCGU--UCAGCu -3' miRNA: 3'- -CACUUUAgUAGGU--GGCGCGgaAGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 47604 | 0.67 | 0.862212 |
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Target: 5'- aUGAAGUCgAUUgAUCGUGCCUgacCGGUa -3' miRNA: 3'- cACUUUAG-UAGgUGGCGCGGAa--GUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 54799 | 0.67 | 0.869706 |
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Target: 5'- aGUGGAG-CAUCCGCCucucaugacuacgGuCGCCUUCAu- -3' miRNA: 3'- -CACUUUaGUAGGUGG-------------C-GCGGAAGUcg -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 1290 | 0.66 | 0.886354 |
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Target: 5'- aUGGGuacg-CCGCCGCGCCcgCAGg -3' miRNA: 3'- cACUUuaguaGGUGGCGCGGaaGUCg -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 51097 | 0.66 | 0.886354 |
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Target: 5'- -----cUCGUCCGCCGCGaggcuguaCCUggaCAGCc -3' miRNA: 3'- cacuuuAGUAGGUGGCGC--------GGAa--GUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 12103 | 0.66 | 0.908005 |
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Target: 5'- cUGcug-CggCUACCGCGgCUUCGGCu -3' miRNA: 3'- cACuuuaGuaGGUGGCGCgGAAGUCG- -5' |
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| 19560 | 5' | -51.2 | NC_004686.1 | + | 55935 | 0.76 | 0.389248 |
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Target: 5'- ------cCAUCgACCGCGCCaUCAGCa -3' miRNA: 3'- cacuuuaGUAGgUGGCGCGGaAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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