Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19571 | 5' | -50.1 | NC_004686.1 | + | 39646 | 0.66 | 0.91204 |
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Target: 5'- uGCUCCGAccGCACAgUGUCAcgggugaggGUGCgGCa -3' miRNA: 3'- cCGAGGUU--CGUGUaACGGU---------UAUGgUG- -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 6496 | 0.66 | 0.923643 |
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Target: 5'- uGGCUCCugcucguagaucGGCugAcccGCCAGaACCGCa -3' miRNA: 3'- -CCGAGGu-----------UCGugUaa-CGGUUaUGGUG- -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 33500 | 0.66 | 0.924872 |
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Target: 5'- uGGCaggCCGAGCACAUggUGCC----CCAg -3' miRNA: 3'- -CCGa--GGUUCGUGUA--ACGGuuauGGUg -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 26307 | 0.66 | 0.924872 |
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Target: 5'- uGGCUgCCGAcaaACAacGCCAAUGCgCACa -3' miRNA: 3'- -CCGA-GGUUcg-UGUaaCGGUUAUG-GUG- -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 19590 | 0.66 | 0.930831 |
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Target: 5'- cGGCUCCGggguaGGCGCAU--CC---ACCGCa -3' miRNA: 3'- -CCGAGGU-----UCGUGUAacGGuuaUGGUG- -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 22490 | 0.66 | 0.93426 |
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Target: 5'- aGGCUgguacagCCAgucgaugcgguuguAGC-CAUcggUGCCGGUGCCGCc -3' miRNA: 3'- -CCGA-------GGU--------------UCGuGUA---ACGGUUAUGGUG- -5' |
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| 19571 | 5' | -50.1 | NC_004686.1 | + | 11676 | 0.66 | 0.936485 |
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Target: 5'- gGGCUUCAAGCccggaGCAUugaaUGUCGGaACCAa -3' miRNA: 3'- -CCGAGGUUCG-----UGUA----ACGGUUaUGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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