Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20411 | 3' | -53 | NC_004688.1 | + | 34377 | 0.66 | 0.958836 |
Target: 5'- gCGGAccGGugaucugcaacAUCAcucccGGCUGGCCGGuCUCCg -3' miRNA: 3'- -GCUUuuCC-----------UAGU-----CCGGCCGGUU-GAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 73547 | 0.66 | 0.958836 |
Target: 5'- aGGccAGGGGGUCcGGCCaGCCgAGCUUUg -3' miRNA: 3'- gCU--UUUCCUAGuCCGGcCGG-UUGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 73853 | 0.66 | 0.958836 |
Target: 5'- uCGggGAGGAUCgcauacuuaccGGG--GGCUAAUUCCc -3' miRNA: 3'- -GCuuUUCCUAG-----------UCCggCCGGUUGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 46210 | 0.66 | 0.958836 |
Target: 5'- uCGAuuGGGAUuacugcagCAGccccaCCGGCCAGCugUCCg -3' miRNA: 3'- -GCUuuUCCUA--------GUCc----GGCCGGUUG--AGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 66616 | 0.66 | 0.958836 |
Target: 5'- aGAGAGuGuGUCgGGGUCGGCCAcgaAUUCCu -3' miRNA: 3'- gCUUUU-CcUAG-UCCGGCCGGU---UGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 29697 | 0.66 | 0.954924 |
Target: 5'- cCGGcuGGGGAUCAcccGGCCG-CCAcaACUUCa -3' miRNA: 3'- -GCUu-UUCCUAGU---CCGGCcGGU--UGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 102366 | 0.66 | 0.954924 |
Target: 5'- gCGGAGcAGG-UguGGCCGGUCuACUgCg -3' miRNA: 3'- -GCUUU-UCCuAguCCGGCCGGuUGAgG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 2174 | 0.66 | 0.954924 |
Target: 5'- aGAGAuggccaagugcGGGGUUGGcGCUGGCgAuCUCCg -3' miRNA: 3'- gCUUU-----------UCCUAGUC-CGGCCGgUuGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 69711 | 0.66 | 0.95452 |
Target: 5'- gGAGuGGGAguUCAccaggguGGCagCGGCCuGCUCCg -3' miRNA: 3'- gCUUuUCCU--AGU-------CCG--GCCGGuUGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 60474 | 0.66 | 0.95452 |
Target: 5'- uCGAAucccucuGGGGAUUcgGGGUCGGCgAcguaguuccagGCUCCu -3' miRNA: 3'- -GCUU-------UUCCUAG--UCCGGCCGgU-----------UGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 20864 | 0.66 | 0.950768 |
Target: 5'- gCGAAcagcGGGuaacccugCAGGCCGcGCCAG-UCCg -3' miRNA: 3'- -GCUUu---UCCua------GUCCGGC-CGGUUgAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 41786 | 0.66 | 0.950768 |
Target: 5'- uGggGuccuccGGGAUCGGGCUG-CCGuCUUCg -3' miRNA: 3'- gCuuU------UCCUAGUCCGGCcGGUuGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 75102 | 0.66 | 0.950768 |
Target: 5'- aCGAAAuGGAaCGGGgUGGCgAccucgcGCUCCg -3' miRNA: 3'- -GCUUUuCCUaGUCCgGCCGgU------UGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 74550 | 0.66 | 0.941707 |
Target: 5'- uGAAGGGGAuUUGGGCCacauccgucacgGGCC--CUCCu -3' miRNA: 3'- gCUUUUCCU-AGUCCGG------------CCGGuuGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 3216 | 0.66 | 0.941707 |
Target: 5'- uCGccAAGGAUgcugccgugCAGGuCCGGCCA-CUCa -3' miRNA: 3'- -GCuuUUCCUA---------GUCC-GGCCGGUuGAGg -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 12903 | 0.66 | 0.941707 |
Target: 5'- cCGAGAAGucUC-GGCCGGgCAgguGCUCUu -3' miRNA: 3'- -GCUUUUCcuAGuCCGGCCgGU---UGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 75450 | 0.66 | 0.936797 |
Target: 5'- cCGGAAAGG-UgGGGCCcGUCGAaUCCg -3' miRNA: 3'- -GCUUUUCCuAgUCCGGcCGGUUgAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 29534 | 0.66 | 0.936797 |
Target: 5'- gCGcAGAGug-CGGGCCacgucgucgaGGCCAAUUCCg -3' miRNA: 3'- -GCuUUUCcuaGUCCGG----------CCGGUUGAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 75348 | 0.67 | 0.93163 |
Target: 5'- gCGAGAAGGAUguugccgaucgcCAGGUCGucGCCA--UCCu -3' miRNA: 3'- -GCUUUUCCUA------------GUCCGGC--CGGUugAGG- -5' |
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20411 | 3' | -53 | NC_004688.1 | + | 31293 | 0.67 | 0.93163 |
Target: 5'- cCGGAGgcGGGGucguUCAGGCgGGCaauCAGgUCCa -3' miRNA: 3'- -GCUUU--UCCU----AGUCCGgCCG---GUUgAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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