Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20421 | 3' | -54 | NC_004688.1 | + | 5344 | 0.66 | 0.93593 |
Target: 5'- cCGCGgGUccACGCuguCGCUguacucguauccgagCGACUCGAGg -3' miRNA: 3'- -GCGCgUA--UGCGu--GCGA---------------GCUGAGCUUg -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 72883 | 0.66 | 0.93389 |
Target: 5'- gGCGCAccgGCuCAUGCUC--CUCGAACc -3' miRNA: 3'- gCGCGUa--UGcGUGCGAGcuGAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 9254 | 0.66 | 0.928612 |
Target: 5'- uCGCGCucuucCGCgGCGCUUucCUCGGGCu -3' miRNA: 3'- -GCGCGuau--GCG-UGCGAGcuGAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 56697 | 0.66 | 0.92807 |
Target: 5'- uCGCGCAUaccgauucugaagACGgAUGC-CGAgcuuCUCGAACu -3' miRNA: 3'- -GCGCGUA-------------UGCgUGCGaGCU----GAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 31257 | 0.66 | 0.925323 |
Target: 5'- aCGCGCGUAUcgGcCGCGC-CGGCgaccugcgucugagCGAACa -3' miRNA: 3'- -GCGCGUAUG--C-GUGCGaGCUGa-------------GCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 19222 | 0.66 | 0.92308 |
Target: 5'- gGCGaguucgGCgGC-CGUUCGugUCGAACg -3' miRNA: 3'- gCGCgua---UG-CGuGCGAGCugAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 95190 | 0.66 | 0.919639 |
Target: 5'- gGCGCAUcccccagcauugcgaGCGCGCGUgagGAaUCGAACc -3' miRNA: 3'- gCGCGUA---------------UGCGUGCGag-CUgAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 24544 | 0.66 | 0.917294 |
Target: 5'- uGCGCAUccgcgACaGCACGCugaUCGACaUCGGu- -3' miRNA: 3'- gCGCGUA-----UG-CGUGCG---AGCUG-AGCUug -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 108739 | 0.66 | 0.916701 |
Target: 5'- aCGuUGCGUACGguCGCgguugCGAgcugggaaccgaaCUCGAGCg -3' miRNA: 3'- -GC-GCGUAUGCguGCGa----GCU-------------GAGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 5939 | 0.66 | 0.911255 |
Target: 5'- cCGCGcCGUGCGCcUGCUUGGcCUUGuACu -3' miRNA: 3'- -GCGC-GUAUGCGuGCGAGCU-GAGCuUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 105952 | 0.66 | 0.911255 |
Target: 5'- cCGCGCGacuUGCGCGaugaGCUgGACgcacgCGAGa -3' miRNA: 3'- -GCGCGU---AUGCGUg---CGAgCUGa----GCUUg -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 100309 | 0.67 | 0.904964 |
Target: 5'- aCGaCGUAcucCGCACGUgccgCGACUgCGGACa -3' miRNA: 3'- -GC-GCGUau-GCGUGCGa---GCUGA-GCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 83542 | 0.67 | 0.891639 |
Target: 5'- uCGCcCAUGCGaucgaGCUUGGCUCGcAGCg -3' miRNA: 3'- -GCGcGUAUGCgug--CGAGCUGAGC-UUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 51417 | 0.68 | 0.861339 |
Target: 5'- aGCGCGccaaggcUGCGCugG-UCGACUaCGAAg -3' miRNA: 3'- gCGCGU-------AUGCGugCgAGCUGA-GCUUg -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 451 | 0.68 | 0.854179 |
Target: 5'- uCGCGCcUGCGguaGCGCUCucGCUCGcGCg -3' miRNA: 3'- -GCGCGuAUGCg--UGCGAGc-UGAGCuUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 106414 | 0.68 | 0.84602 |
Target: 5'- gCGCGCGUAUcgcuaGCGCGC-CGGCUagUGAGg -3' miRNA: 3'- -GCGCGUAUG-----CGUGCGaGCUGA--GCUUg -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 10541 | 0.68 | 0.837655 |
Target: 5'- -uCGCGUAgGUGCGCcCGGCgCGAGCg -3' miRNA: 3'- gcGCGUAUgCGUGCGaGCUGaGCUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 67717 | 0.68 | 0.837655 |
Target: 5'- cCGCGuCGUACuCGCGCUCGGCcCGc-- -3' miRNA: 3'- -GCGC-GUAUGcGUGCGAGCUGaGCuug -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 20485 | 0.68 | 0.829091 |
Target: 5'- aGCGCAcg-GCGCGCUUG-CUCuGGGCg -3' miRNA: 3'- gCGCGUaugCGUGCGAGCuGAG-CUUG- -5' |
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20421 | 3' | -54 | NC_004688.1 | + | 36341 | 0.68 | 0.828224 |
Target: 5'- cCGCGCAaacuucgGCGCggccucgaaccccAgGCUCGGCgCGAACg -3' miRNA: 3'- -GCGCGUa------UGCG-------------UgCGAGCUGaGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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