Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20421 | 3' | -54 | NC_004688.1 | + | 80226 | 0.69 | 0.802292 |
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Target: 5'- uCG-GCGUACGCcCGCUuaucCGACUCGGc- -3' miRNA: 3'- -GCgCGUAUGCGuGCGA----GCUGAGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 99608 | 0.7 | 0.764315 |
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Target: 5'- gGCugGCGUGCGcCACGC-CGAgaaaCUCGGACu -3' miRNA: 3'- gCG--CGUAUGC-GUGCGaGCU----GAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 62732 | 0.7 | 0.724343 |
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Target: 5'- cCGCGacugGUGCGCACGCaagGAUUUGAACc -3' miRNA: 3'- -GCGCg---UAUGCGUGCGag-CUGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 31655 | 0.72 | 0.651525 |
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Target: 5'- cCGCGCAggGCGgACaGCUCGGC-CGAGg -3' miRNA: 3'- -GCGCGUa-UGCgUG-CGAGCUGaGCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 4068 | 0.73 | 0.567441 |
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Target: 5'- cCGCgGCGUAgGCGCGCaUCGuCUCGAu- -3' miRNA: 3'- -GCG-CGUAUgCGUGCG-AGCuGAGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 70217 | 0.75 | 0.496207 |
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Target: 5'- aGCGCuccgccggGCGUGCGCUUGAUUCGAu- -3' miRNA: 3'- gCGCGua------UGCGUGCGAGCUGAGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 103472 | 0.75 | 0.44788 |
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Target: 5'- cCGCGaAUACGCGCGacagaUCGAgUCGGGCg -3' miRNA: 3'- -GCGCgUAUGCGUGCg----AGCUgAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 101891 | 0.77 | 0.393516 |
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Target: 5'- gCGCGCAUAC-CACGCcCGACUgGGAa -3' miRNA: 3'- -GCGCGUAUGcGUGCGaGCUGAgCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 11273 | 1.11 | 0.002549 |
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Target: 5'- gCGCGCAUACGCACGCUCGACUCGAACa -3' miRNA: 3'- -GCGCGUAUGCGUGCGAGCUGAGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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