Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20421 | 3' | -54 | NC_004688.1 | + | 31655 | 0.72 | 0.651525 |
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Target: 5'- cCGCGCAggGCGgACaGCUCGGC-CGAGg -3' miRNA: 3'- -GCGCGUa-UGCgUG-CGAGCUGaGCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 62732 | 0.7 | 0.724343 |
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Target: 5'- cCGCGacugGUGCGCACGCaagGAUUUGAACc -3' miRNA: 3'- -GCGCg---UAUGCGUGCGag-CUGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 99608 | 0.7 | 0.764315 |
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Target: 5'- gGCugGCGUGCGcCACGC-CGAgaaaCUCGGACu -3' miRNA: 3'- gCG--CGUAUGC-GUGCGaGCU----GAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 80226 | 0.69 | 0.802292 |
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Target: 5'- uCG-GCGUACGCcCGCUuaucCGACUCGGc- -3' miRNA: 3'- -GCgCGUAUGCGuGCGA----GCUGAGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 36341 | 0.68 | 0.828224 |
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Target: 5'- cCGCGCAaacuucgGCGCggccucgaaccccAgGCUCGGCgCGAACg -3' miRNA: 3'- -GCGCGUa------UGCG-------------UgCGAGCUGaGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 20485 | 0.68 | 0.829091 |
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Target: 5'- aGCGCAcg-GCGCGCUUG-CUCuGGGCg -3' miRNA: 3'- gCGCGUaugCGUGCGAGCuGAG-CUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 67717 | 0.68 | 0.837655 |
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Target: 5'- cCGCGuCGUACuCGCGCUCGGCcCGc-- -3' miRNA: 3'- -GCGC-GUAUGcGUGCGAGCUGaGCuug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 10541 | 0.68 | 0.837655 |
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Target: 5'- -uCGCGUAgGUGCGCcCGGCgCGAGCg -3' miRNA: 3'- gcGCGUAUgCGUGCGaGCUGaGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 5344 | 0.66 | 0.93593 |
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Target: 5'- cCGCGgGUccACGCuguCGCUguacucguauccgagCGACUCGAGg -3' miRNA: 3'- -GCGCgUA--UGCGu--GCGA---------------GCUGAGCUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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