Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20421 | 3' | -54 | NC_004688.1 | + | 108739 | 0.66 | 0.916701 |
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Target: 5'- aCGuUGCGUACGguCGCgguugCGAgcugggaaccgaaCUCGAGCg -3' miRNA: 3'- -GC-GCGUAUGCguGCGa----GCU-------------GAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 24544 | 0.66 | 0.917294 |
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Target: 5'- uGCGCAUccgcgACaGCACGCugaUCGACaUCGGu- -3' miRNA: 3'- gCGCGUA-----UG-CGUGCG---AGCUG-AGCUug -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 95190 | 0.66 | 0.919639 |
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Target: 5'- gGCGCAUcccccagcauugcgaGCGCGCGUgagGAaUCGAACc -3' miRNA: 3'- gCGCGUA---------------UGCGUGCGag-CUgAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 19222 | 0.66 | 0.92308 |
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Target: 5'- gGCGaguucgGCgGC-CGUUCGugUCGAACg -3' miRNA: 3'- gCGCgua---UG-CGuGCGAGCugAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 31257 | 0.66 | 0.925323 |
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Target: 5'- aCGCGCGUAUcgGcCGCGC-CGGCgaccugcgucugagCGAACa -3' miRNA: 3'- -GCGCGUAUG--C-GUGCGaGCUGa-------------GCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 56697 | 0.66 | 0.92807 |
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Target: 5'- uCGCGCAUaccgauucugaagACGgAUGC-CGAgcuuCUCGAACu -3' miRNA: 3'- -GCGCGUA-------------UGCgUGCGaGCU----GAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 9254 | 0.66 | 0.928612 |
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Target: 5'- uCGCGCucuucCGCgGCGCUUucCUCGGGCu -3' miRNA: 3'- -GCGCGuau--GCG-UGCGAGcuGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 72883 | 0.66 | 0.93389 |
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Target: 5'- gGCGCAccgGCuCAUGCUC--CUCGAACc -3' miRNA: 3'- gCGCGUa--UGcGUGCGAGcuGAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 5344 | 0.66 | 0.93593 |
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Target: 5'- cCGCGgGUccACGCuguCGCUguacucguauccgagCGACUCGAGg -3' miRNA: 3'- -GCGCgUA--UGCGu--GCGA---------------GCUGAGCUUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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