Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20421 | 3' | -54 | NC_004688.1 | + | 83542 | 0.67 | 0.891639 |
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Target: 5'- uCGCcCAUGCGaucgaGCUUGGCUCGcAGCg -3' miRNA: 3'- -GCGcGUAUGCgug--CGAGCUGAGC-UUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 95190 | 0.66 | 0.919639 |
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Target: 5'- gGCGCAUcccccagcauugcgaGCGCGCGUgagGAaUCGAACc -3' miRNA: 3'- gCGCGUA---------------UGCGUGCGag-CUgAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 99608 | 0.7 | 0.764315 |
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Target: 5'- gGCugGCGUGCGcCACGC-CGAgaaaCUCGGACu -3' miRNA: 3'- gCG--CGUAUGC-GUGCGaGCU----GAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 100309 | 0.67 | 0.904964 |
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Target: 5'- aCGaCGUAcucCGCACGUgccgCGACUgCGGACa -3' miRNA: 3'- -GC-GCGUau-GCGUGCGa---GCUGA-GCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 101891 | 0.77 | 0.393516 |
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Target: 5'- gCGCGCAUAC-CACGCcCGACUgGGAa -3' miRNA: 3'- -GCGCGUAUGcGUGCGaGCUGAgCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 103472 | 0.75 | 0.44788 |
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Target: 5'- cCGCGaAUACGCGCGacagaUCGAgUCGGGCg -3' miRNA: 3'- -GCGCgUAUGCGUGCg----AGCUgAGCUUG- -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 105952 | 0.66 | 0.911255 |
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Target: 5'- cCGCGCGacuUGCGCGaugaGCUgGACgcacgCGAGa -3' miRNA: 3'- -GCGCGU---AUGCGUg---CGAgCUGa----GCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 106414 | 0.68 | 0.84602 |
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Target: 5'- gCGCGCGUAUcgcuaGCGCGC-CGGCUagUGAGg -3' miRNA: 3'- -GCGCGUAUG-----CGUGCGaGCUGA--GCUUg -5' |
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| 20421 | 3' | -54 | NC_004688.1 | + | 108739 | 0.66 | 0.916701 |
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Target: 5'- aCGuUGCGUACGguCGCgguugCGAgcugggaaccgaaCUCGAGCg -3' miRNA: 3'- -GC-GCGUAUGCguGCGa----GCU-------------GAGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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