Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20543 | 5' | -60.4 | NC_004688.1 | + | 91351 | 0.7 | 0.449657 |
Target: 5'- aGGcCCGCGAUcUGCUugcuGCCgCGGCgCCa -3' miRNA: 3'- cCCaGGCGCUA-ACGGu---CGG-GCCGaGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 64653 | 0.69 | 0.477077 |
Target: 5'- --cUCCG-GAgaGCCAgugaagggugucGCCCGGCUCCa -3' miRNA: 3'- cccAGGCgCUaaCGGU------------CGGGCCGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 87926 | 0.68 | 0.534215 |
Target: 5'- --aUCCGCGAccGCaGGCCCG-CUCCa -3' miRNA: 3'- cccAGGCGCUaaCGgUCGGGCcGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 27548 | 0.68 | 0.543986 |
Target: 5'- gGGGUCCaGCGGgc-CCGGCCgGGgaUCa -3' miRNA: 3'- -CCCAGG-CGCUaacGGUCGGgCCgaGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 52728 | 0.68 | 0.553815 |
Target: 5'- --aUCCGCGAaUGCUggGGgCCGGaCUCCc -3' miRNA: 3'- cccAGGCGCUaACGG--UCgGGCC-GAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 5162 | 0.68 | 0.557761 |
Target: 5'- cGGG-CaCGCGGUaGCgguagauguucgaauCGGCCCGGC-CCg -3' miRNA: 3'- -CCCaG-GCGCUAaCG---------------GUCGGGCCGaGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 58394 | 0.66 | 0.683964 |
Target: 5'- uGGGcaCCGCGGUgugGCUGG-CgGGCUUCg -3' miRNA: 3'- -CCCa-GGCGCUAa--CGGUCgGgCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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