Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20543 | 5' | -60.4 | NC_004688.1 | + | 3487 | 0.66 | 0.653923 |
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Target: 5'- uGGUCgGCGGcuugcugauggUGCCAGCCgaGGcCUCUu -3' miRNA: 3'- cCCAGgCGCUa----------ACGGUCGGg-CC-GAGG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 9427 | 0.66 | 0.653923 |
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Target: 5'- cGGUCuCGCGGaa-CCAucGCUCGGCUUCu -3' miRNA: 3'- cCCAG-GCGCUaacGGU--CGGGCCGAGG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 4622 | 0.66 | 0.660954 |
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Target: 5'- cGGGUCgagaaugaggccgcaCGCGGcgGCCAggucaaucgcuuccuGCCCGGCagaggauuuagcuUCCg -3' miRNA: 3'- -CCCAG---------------GCGCUaaCGGU---------------CGGGCCG-------------AGG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 24586 | 0.66 | 0.663964 |
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Target: 5'- cGGGcgUCGCGAagGUCgAGCgCGGCgcgCCg -3' miRNA: 3'- -CCCa-GGCGCUaaCGG-UCGgGCCGa--GG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 18090 | 0.66 | 0.680973 |
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Target: 5'- uGGGcCCGCGGcaccaccagccGCacuucuuauagagCAGCCgGGCUCCc -3' miRNA: 3'- -CCCaGGCGCUaa---------CG-------------GUCGGgCCGAGG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 86797 | 0.66 | 0.683964 |
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Target: 5'- -cGUCC-CGAacgggaccGCCGGuCCCGGCUUCu -3' miRNA: 3'- ccCAGGcGCUaa------CGGUC-GGGCCGAGG- -5' |
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| 20543 | 5' | -60.4 | NC_004688.1 | + | 58394 | 0.66 | 0.683964 |
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Target: 5'- uGGGcaCCGCGGUgugGCUGG-CgGGCUUCg -3' miRNA: 3'- -CCCa-GGCGCUAa--CGGUCgGgCCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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