Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20543 | 5' | -60.4 | NC_004688.1 | + | 87273 | 0.72 | 0.312724 |
Target: 5'- uGGauaCGgGAUUGCC-GCCgGGCUCCa -3' miRNA: 3'- cCCag-GCgCUAACGGuCGGgCCGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 87926 | 0.68 | 0.534215 |
Target: 5'- --aUCCGCGAccGCaGGCCCG-CUCCa -3' miRNA: 3'- cccAGGCGCUaaCGgUCGGGCcGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 89792 | 0.67 | 0.593591 |
Target: 5'- cGGGgCCGC----GCCAGCCgggaGGCUUCu -3' miRNA: 3'- -CCCaGGCGcuaaCGGUCGGg---CCGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 91351 | 0.7 | 0.449657 |
Target: 5'- aGGcCCGCGAUcUGCUugcuGCCgCGGCgCCa -3' miRNA: 3'- cCCaGGCGCUA-ACGGu---CGG-GCCGaGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 91851 | 1.13 | 0.000472 |
Target: 5'- aGGGUCCGCGAUUGCCAGCCCGGCUCCu -3' miRNA: 3'- -CCCAGGCGCUAACGGUCGGGCCGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 102175 | 0.66 | 0.653923 |
Target: 5'- uGGGaaCCGUGGga-UCAGCCCGaggcGCUCCa -3' miRNA: 3'- -CCCa-GGCGCUaacGGUCGGGC----CGAGG- -5' |
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20543 | 5' | -60.4 | NC_004688.1 | + | 108729 | 0.67 | 0.593591 |
Target: 5'- cGGU-CGCGGUUGCgAGCUgGGaaCCg -3' miRNA: 3'- cCCAgGCGCUAACGgUCGGgCCgaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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