Results 1 - 20 of 90 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2059 | 3' | -57 | NC_001348.1 | + | 121461 | 0.66 | 0.867186 |
Target: 5'- aCUCCGuCCCCCGAUgCAugcgcaugACCCgCu -3' miRNA: 3'- -GGGGC-GGGGGUUGgGUuuua----UGGGgG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 109 | 0.66 | 0.867186 |
Target: 5'- cCCCCcCCCgCCAGCCUuuAAcaaaACCCgCg -3' miRNA: 3'- -GGGGcGGG-GGUUGGGuuUUa---UGGGgG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 92492 | 0.66 | 0.867186 |
Target: 5'- gUUCGUaaUCCCAACCCAcauggACCUCCu -3' miRNA: 3'- gGGGCG--GGGGUUGGGUuuua-UGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 108331 | 0.66 | 0.867186 |
Target: 5'- aCUCCGuCCCCCGAUgCAugcgcaugACCCgCu -3' miRNA: 3'- -GGGGC-GGGGGUUGgGUuuua----UGGGgG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 338 | 0.66 | 0.866443 |
Target: 5'- uCCCCGCCUCUuuuuCCUcacguggAGAAUggcACCaCCCa -3' miRNA: 3'- -GGGGCGGGGGuu--GGG-------UUUUA---UGG-GGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 108845 | 0.66 | 0.859665 |
Target: 5'- uUCCCGUCCCa---CCAug--ACCCCg -3' miRNA: 3'- -GGGGCGGGGguugGGUuuuaUGGGGg -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 120946 | 0.66 | 0.859665 |
Target: 5'- uUCCCGUCCCa---CCAug--ACCCCg -3' miRNA: 3'- -GGGGCGGGGguugGGUuuuaUGGGGg -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 111246 | 0.66 | 0.859665 |
Target: 5'- uCCCCGUCUCgauaaCAAUCgCcGGGUACCCCg -3' miRNA: 3'- -GGGGCGGGG-----GUUGG-GuUUUAUGGGGg -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 118546 | 0.66 | 0.859665 |
Target: 5'- uCCCCGUCUCgauaaCAAUCgCcGGGUACCCCg -3' miRNA: 3'- -GGGGCGGGG-----GUUGG-GuUUUAUGGGGg -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 114986 | 0.66 | 0.851941 |
Target: 5'- aCCCGCgacaCCCAAagggUCCG--GUACCUCCc -3' miRNA: 3'- gGGGCGg---GGGUU----GGGUuuUAUGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 106751 | 0.66 | 0.851941 |
Target: 5'- ---gGCCCCCcggggaauACgCCGAAAU-CCCCCg -3' miRNA: 3'- ggggCGGGGGu-------UG-GGUUUUAuGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 123041 | 0.66 | 0.851941 |
Target: 5'- ---gGCCCCCcggggaauACgCCGAAAU-CCCCCg -3' miRNA: 3'- ggggCGGGGGu-------UG-GGUUUUAuGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 2531 | 0.66 | 0.851941 |
Target: 5'- aUCCCGCccauauauCCCCAacgucGCCUuAAAaACUCCCa -3' miRNA: 3'- -GGGGCG--------GGGGU-----UGGGuUUUaUGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 70093 | 0.66 | 0.841606 |
Target: 5'- aUCUCGCCUCCAugUuugCGGGGUAuggaaauuaagcauCCCCCg -3' miRNA: 3'- -GGGGCGGGGGUugG---GUUUUAU--------------GGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 124702 | 0.66 | 0.839987 |
Target: 5'- aCUCCGucaCCCCCGACUCugcggggggcuccuCCCCCc -3' miRNA: 3'- -GGGGC---GGGGGUUGGGuuuuau--------GGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 105090 | 0.66 | 0.839987 |
Target: 5'- aCUCCGucaCCCCCGACUCugcggggggcuccuCCCCCc -3' miRNA: 3'- -GGGGC---GGGGGUUGGGuuuuau--------GGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 68584 | 0.66 | 0.839175 |
Target: 5'- aCCCCacuaccgccagaaacGCCCCU--CCUAAAGagGCCCCa -3' miRNA: 3'- -GGGG---------------CGGGGGuuGGGUUUUa-UGGGGg -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 9296 | 0.67 | 0.827614 |
Target: 5'- gCCCGUguugaCCCCAaaacagACgCAAGAUguacaacgcacaACCCCCg -3' miRNA: 3'- gGGGCG-----GGGGU------UGgGUUUUA------------UGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 117238 | 0.67 | 0.827614 |
Target: 5'- aCCgGCgaCUacuaAACCCAAGGaaauUACCCCCg -3' miRNA: 3'- gGGgCGg-GGg---UUGGGUUUU----AUGGGGG- -5' |
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2059 | 3' | -57 | NC_001348.1 | + | 74257 | 0.67 | 0.819144 |
Target: 5'- --gCGCCCCCGAUgCGGGA-GCCgCCa -3' miRNA: 3'- gggGCGGGGGUUGgGUUUUaUGGgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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