Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20591 | 5' | -61 | NC_004688.1 | + | 88411 | 0.67 | 0.500779 |
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Target: 5'- --aGCCUGaGCUGCUugAuucgaUGGUCGGCg -3' miRNA: 3'- ucgCGGACgCGGCGGugU-----ACCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 92668 | 0.75 | 0.167821 |
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Target: 5'- uGGCGCCgGUGCCGaCCAUcaGGUUGGCg -3' miRNA: 3'- -UCGCGGaCGCGGC-GGUGuaCCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 93815 | 0.67 | 0.500779 |
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Target: 5'- uGGCGaCCU-CGCCGCCGuuUGGgccCGACc -3' miRNA: 3'- -UCGC-GGAcGCGGCGGUguACCa--GCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 95657 | 0.67 | 0.510429 |
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Target: 5'- cGGCGCC-GCGCCGCggCACAUGaaagguGUCcagGGCa -3' miRNA: 3'- -UCGCGGaCGCGGCG--GUGUAC------CAG---CUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 96056 | 0.77 | 0.129985 |
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Target: 5'- cGCGaCUGgGCCuGCCGCAcGGUCGACg -3' miRNA: 3'- uCGCgGACgCGG-CGGUGUaCCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 96946 | 0.68 | 0.444724 |
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Target: 5'- cGCGCUccguaucguugGCGCUGCCGCAcagcgUGG-CGGCg -3' miRNA: 3'- uCGCGGa----------CGCGGCGGUGU-----ACCaGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 100167 | 0.66 | 0.610266 |
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Target: 5'- --gGCCUGCacgaaGCCGUC-CGUcaGGUCGACc -3' miRNA: 3'- ucgCGGACG-----CGGCGGuGUA--CCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 100790 | 0.66 | 0.610266 |
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Target: 5'- uGGCGCCgugGCGaUCGCCugAcGcUCGGCc -3' miRNA: 3'- -UCGCGGa--CGC-GGCGGugUaCcAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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