Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20591 | 5' | -61 | NC_004688.1 | + | 70885 | 0.66 | 0.569764 |
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Target: 5'- uGGCGUC--CGCCGCCGCAgUGG-CGGg -3' miRNA: 3'- -UCGCGGacGCGGCGGUGU-ACCaGCUg -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 184 | 0.66 | 0.569764 |
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Target: 5'- uGGCGaUUGCGaCCGCCugagagccACGUGGagCGACg -3' miRNA: 3'- -UCGCgGACGC-GGCGG--------UGUACCa-GCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 85701 | 0.66 | 0.578831 |
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Target: 5'- cGCGCCguuuccGUGCuCGCCguacuggucgggcGCGUGGUCGcCc -3' miRNA: 3'- uCGCGGa-----CGCG-GCGG-------------UGUACCAGCuG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 85132 | 0.66 | 0.589954 |
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Target: 5'- gGGcCGCCUuggGgGCCGCCGgAgcgaguggGGUCGAg -3' miRNA: 3'- -UC-GCGGA---CgCGGCGGUgUa-------CCAGCUg -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 31930 | 0.66 | 0.589954 |
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Target: 5'- -cCGCUga-GCUGCCGCGgacGGUCGGCu -3' miRNA: 3'- ucGCGGacgCGGCGGUGUa--CCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 85040 | 0.66 | 0.600098 |
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Target: 5'- cGGCGUUggUGCGCaGCgACG-GGUCGAUg -3' miRNA: 3'- -UCGCGG--ACGCGgCGgUGUaCCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 100167 | 0.66 | 0.610266 |
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Target: 5'- --gGCCUGCacgaaGCCGUC-CGUcaGGUCGACc -3' miRNA: 3'- ucgCGGACG-----CGGCGGuGUA--CCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 100790 | 0.66 | 0.610266 |
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Target: 5'- uGGCGCCgugGCGaUCGCCugAcGcUCGGCc -3' miRNA: 3'- -UCGCGGa--CGC-GGCGGugUaCcAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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