Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 20591 | 5' | -61 | NC_004688.1 | + | 30803 | 0.7 | 0.37582 |
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Target: 5'- uGCGCC-GCGUgGCCACGUGGgugccaugcCGAa -3' miRNA: 3'- uCGCGGaCGCGgCGGUGUACCa--------GCUg -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 67567 | 0.7 | 0.379091 |
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Target: 5'- uGCGCCUGCaaaggcggcacacccGgCCGCCAUcgGGggCGAUg -3' miRNA: 3'- uCGCGGACG---------------C-GGCGGUGuaCCa-GCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 60806 | 0.7 | 0.384032 |
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Target: 5'- cGCGCCUGCGUCGauguggCGCcgGaUCGACc -3' miRNA: 3'- uCGCGGACGCGGCg-----GUGuaCcAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 87541 | 0.68 | 0.444724 |
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Target: 5'- cGCGCCgGCGauGCC-CGUGGUCaGCc -3' miRNA: 3'- uCGCGGaCGCggCGGuGUACCAGcUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 96946 | 0.68 | 0.444724 |
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Target: 5'- cGCGCUccguaucguugGCGCUGCCGCAcagcgUGG-CGGCg -3' miRNA: 3'- uCGCGGa----------CGCGGCGGUGU-----ACCaGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 33899 | 0.68 | 0.46674 |
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Target: 5'- gGGUGCCgcaccccaucugaaGCGCuCGCCAUcgGGUCaACa -3' miRNA: 3'- -UCGCGGa-------------CGCG-GCGGUGuaCCAGcUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 25606 | 0.68 | 0.481726 |
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Target: 5'- uGGCgGCCaGCGCCGCCugGUGcUCu-- -3' miRNA: 3'- -UCG-CGGaCGCGGCGGugUACcAGcug -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 100790 | 0.66 | 0.610266 |
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Target: 5'- uGGCGCCgugGCGaUCGCCugAcGcUCGGCc -3' miRNA: 3'- -UCGCGGa--CGC-GGCGGugUaCcAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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