Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20591 | 5' | -61 | NC_004688.1 | + | 67567 | 0.7 | 0.379091 |
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Target: 5'- uGCGCCUGCaaaggcggcacacccGgCCGCCAUcgGGggCGAUg -3' miRNA: 3'- uCGCGGACG---------------C-GGCGGUGuaCCa-GCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 30803 | 0.7 | 0.37582 |
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Target: 5'- uGCGCC-GCGUgGCCACGUGGgugccaugcCGAa -3' miRNA: 3'- uCGCGGaCGCGgCGGUGUACCa--------GCUg -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 88031 | 0.72 | 0.267302 |
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Target: 5'- cGCGCUUGCGCCGCUucuCAUucUCGGCc -3' miRNA: 3'- uCGCGGACGCGGCGGu--GUAccAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 70991 | 0.72 | 0.261047 |
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Target: 5'- cGGCGCCUGCuCCGCCGgGUccGGU-GGCg -3' miRNA: 3'- -UCGCGGACGcGGCGGUgUA--CCAgCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 92668 | 0.75 | 0.167821 |
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Target: 5'- uGGCGCCgGUGCCGaCCAUcaGGUUGGCg -3' miRNA: 3'- -UCGCGGaCGCGGC-GGUGuaCCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 71666 | 0.77 | 0.133383 |
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Target: 5'- cAGCGCCUGgGuuaCCGCCGguuUGGUCGGCg -3' miRNA: 3'- -UCGCGGACgC---GGCGGUgu-ACCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 96056 | 0.77 | 0.129985 |
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Target: 5'- cGCGaCUGgGCCuGCCGCAcGGUCGACg -3' miRNA: 3'- uCGCgGACgCGG-CGGUGUaCCAGCUG- -5' |
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| 20591 | 5' | -61 | NC_004688.1 | + | 75361 | 0.78 | 0.102829 |
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Target: 5'- -cCGCCgGCGCCGCCAUauGUGGcCGACg -3' miRNA: 3'- ucGCGGaCGCGGCGGUG--UACCaGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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