Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2066 | 3' | -48.4 | NC_001348.1 | + | 121757 | 0.71 | 0.96059 |
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Target: 5'- cCGGCGcGUuucgguGGGAAGUGAAACUacagGCa -3' miRNA: 3'- -GCCGUaCAuu----CCCUUCAUUUUGGg---CG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 108034 | 0.71 | 0.96059 |
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Target: 5'- cCGGCGcGUuucgguGGGAAGUGAAACUacagGCa -3' miRNA: 3'- -GCCGUaCAuu----CCCUUCAUUUUGGg---CG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 80874 | 0.71 | 0.952526 |
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Target: 5'- uGGCguugGUGUAAGaucugcGGggGUGGGAUCUGCu -3' miRNA: 3'- gCCG----UACAUUC------CCuuCAUUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 65933 | 0.72 | 0.933251 |
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Target: 5'- uGGUggGUGAGGaGggGUAAAGgCaCGCu -3' miRNA: 3'- gCCGuaCAUUCC-CuuCAUUUUgG-GCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 86782 | 0.72 | 0.921996 |
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Target: 5'- cCGaGCggGUAcGGGGAuacauacgaaaAGUAAAACCUGCa -3' miRNA: 3'- -GC-CGuaCAU-UCCCU-----------UCAUUUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 90607 | 0.74 | 0.881794 |
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Target: 5'- aGGCAUG--GGGGGAGUGuu-UCUGCg -3' miRNA: 3'- gCCGUACauUCCCUUCAUuuuGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 35964 | 0.75 | 0.814463 |
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Target: 5'- uCGGCAUGUAAagcgccGGuuGUAuccuGAACCCGCa -3' miRNA: 3'- -GCCGUACAUUc-----CCuuCAU----UUUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 98958 | 0.76 | 0.766003 |
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Target: 5'- aGuaAUGUGGGGGggGUAcuAUCCGUa -3' miRNA: 3'- gCcgUACAUUCCCuuCAUuuUGGGCG- -5' |
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| 2066 | 3' | -48.4 | NC_001348.1 | + | 103267 | 1.14 | 0.006379 |
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Target: 5'- uCGGCAUGUAAGGGAAGUAAAACCCGCa -3' miRNA: 3'- -GCCGUACAUUCCCUUCAUUUUGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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