Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21183 | 5' | -47.6 | NC_004778.3 | + | 34978 | 0.7 | 0.987469 |
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Target: 5'- aGCGGcacgucgcUUGU-GUCGAACauuUGCAGCGGa -3' miRNA: 3'- cCGCC--------AAUAuUAGUUUGg--ACGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 76330 | 0.71 | 0.974411 |
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Target: 5'- cGGCGGUugcUGUGGcggCGGuugCUGCGGCGGc -3' miRNA: 3'- -CCGCCA---AUAUUa--GUUug-GACGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 121241 | 0.71 | 0.971518 |
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Target: 5'- uGCGGcg--AGUCgGggUCUGCGGCGGg -3' miRNA: 3'- cCGCCaauaUUAG-UuuGGACGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 63495 | 0.72 | 0.968396 |
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Target: 5'- gGGCGGaUUcgGGcUC-AugCUGCGGCGGc -3' miRNA: 3'- -CCGCC-AAuaUU-AGuUugGACGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 90234 | 0.72 | 0.967414 |
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Target: 5'- aGGCGGUcg-AGUCGcuuagcgccugcauGACC-GUAGCGGg -3' miRNA: 3'- -CCGCCAauaUUAGU--------------UUGGaCGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 85728 | 0.72 | 0.957591 |
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Target: 5'- cGGCGcccccGUAAUUAAGCUUGCAGCa- -3' miRNA: 3'- -CCGCcaa--UAUUAGUUUGGACGUCGcc -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 76533 | 0.75 | 0.897778 |
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Target: 5'- cGGCGacGUUGUAAUCGuACCUGUAGUu- -3' miRNA: 3'- -CCGC--CAAUAUUAGUuUGGACGUCGcc -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 76450 | 0.75 | 0.875874 |
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Target: 5'- cGGCGGUUGcGGcgGGGCCUGCGGCc- -3' miRNA: 3'- -CCGCCAAUaUUagUUUGGACGUCGcc -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 81799 | 0.78 | 0.768142 |
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Target: 5'- gGGCGGcgucUGUAAcCAGAacuCCUGCGGCGGc -3' miRNA: 3'- -CCGCCa---AUAUUaGUUU---GGACGUCGCC- -5' |
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| 21183 | 5' | -47.6 | NC_004778.3 | + | 127541 | 1.14 | 0.008351 |
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Target: 5'- cGGCGGUUAUAAUCAAACCUGCAGCGGc -3' miRNA: 3'- -CCGCCAAUAUUAGUUUGGACGUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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