Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21189 | 5' | -61.9 | NC_004778.3 | + | 8466 | 0.66 | 0.646487 |
Target: 5'- -gGCGgCGCCGGGGCaGCGGgCGcGg -3' miRNA: 3'- gaCGCgGCGGCUCUGcUGCCgGCaCa -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 109123 | 0.66 | 0.636601 |
Target: 5'- uUGUGCCGCCGAuggugccguuaGAcCGuACGGCCa--- -3' miRNA: 3'- gACGCGGCGGCU-----------CU-GC-UGCCGGcaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 97001 | 0.66 | 0.626711 |
Target: 5'- gCUGCGCCGCaugcuGAUG-CaGCCGUGc -3' miRNA: 3'- -GACGCGGCGgcu--CUGCuGcCGGCACa -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 1640 | 0.66 | 0.616825 |
Target: 5'- gCUGCGCUGCgGAaaGCGACGcGUCGUu- -3' miRNA: 3'- -GACGCGGCGgCUc-UGCUGC-CGGCAca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 33366 | 0.66 | 0.597092 |
Target: 5'- -gGCGaCGUCGcGGACGACGcGCCGUc- -3' miRNA: 3'- gaCGCgGCGGC-UCUGCUGC-CGGCAca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 111387 | 0.67 | 0.591189 |
Target: 5'- gCUGCGaggugcucaCCagcauacguuugaggGCCGGGAUGAgGGCUGUGUu -3' miRNA: 3'- -GACGC---------GG---------------CGGCUCUGCUgCCGGCACA- -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 14566 | 0.67 | 0.587259 |
Target: 5'- gUGCGCCaGCauuGugGACGGCC-UGg -3' miRNA: 3'- gACGCGG-CGgcuCugCUGCCGGcACa -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 1520 | 0.67 | 0.577455 |
Target: 5'- -gGCGCUGCCGAGGu--CGGCCa--- -3' miRNA: 3'- gaCGCGGCGGCUCUgcuGCCGGcaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 17245 | 0.67 | 0.567689 |
Target: 5'- aUGCGCgagggCGCCGAGaACGAagugcaguCGGCCGa-- -3' miRNA: 3'- gACGCG-----GCGGCUC-UGCU--------GCCGGCaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 10706 | 0.67 | 0.557964 |
Target: 5'- gUGCGCCGCCGcGcCGA-GGCCa--- -3' miRNA: 3'- gACGCGGCGGCuCuGCUgCCGGcaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 81784 | 0.67 | 0.557964 |
Target: 5'- gUGCGCggccUGCCGGGGCGGCGuCUGUa- -3' miRNA: 3'- gACGCG----GCGGCUCUGCUGCcGGCAca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 32055 | 0.67 | 0.548287 |
Target: 5'- -cGCGCCGCCacacGAUGGCGGUCa--- -3' miRNA: 3'- gaCGCGGCGGcu--CUGCUGCCGGcaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 100241 | 0.67 | 0.545394 |
Target: 5'- aCUGCcgcgugcgcgauugGCUGCCGAG-CGACGacCCGUGg -3' miRNA: 3'- -GACG--------------CGGCGGCUCuGCUGCc-GGCACa -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 121540 | 0.68 | 0.526242 |
Target: 5'- -aGCGCCGCCG-GccagcaacacgcccACGcCGGCCGUu- -3' miRNA: 3'- gaCGCGGCGGCuC--------------UGCuGCCGGCAca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 5547 | 0.68 | 0.519597 |
Target: 5'- gCUGUGCCucuuCCGcaAGugGAuaUGGCCGUGUu -3' miRNA: 3'- -GACGCGGc---GGC--UCugCU--GCCGGCACA- -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 88161 | 0.68 | 0.510163 |
Target: 5'- -cGCGCuCGCCaAGAUGACGGacguCGUGc -3' miRNA: 3'- gaCGCG-GCGGcUCUGCUGCCg---GCACa -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 61295 | 0.68 | 0.500802 |
Target: 5'- aUGCGCCGUCGccGACGugcgagaguACGGCCGc-- -3' miRNA: 3'- gACGCGGCGGCu-CUGC---------UGCCGGCaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 10639 | 0.68 | 0.498008 |
Target: 5'- -gGCGCCGCUGGGccucguaauugugcGCGcCGGCCGc-- -3' miRNA: 3'- gaCGCGGCGGCUC--------------UGCuGCCGGCaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 114362 | 0.68 | 0.491517 |
Target: 5'- cCUGCG-CGCCGcGGGCGA-GGCCGg-- -3' miRNA: 3'- -GACGCgGCGGC-UCUGCUgCCGGCaca -5' |
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21189 | 5' | -61.9 | NC_004778.3 | + | 49434 | 0.68 | 0.491517 |
Target: 5'- uUGCGCCGCCGAcGcagccucuuGCGAgGGCaCGUu- -3' miRNA: 3'- gACGCGGCGGCU-C---------UGCUgCCG-GCAca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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