Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21204 | 3' | -52.2 | NC_004778.3 | + | 118694 | 0.66 | 0.969409 |
Target: 5'- gGCGGCGCGcacacGGCagcccgcgGAGCGCgcAGCGAa -3' miRNA: 3'- aUGUCGUGC-----CCGaa------UUUGCGa-UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 29336 | 0.66 | 0.969409 |
Target: 5'- cGCGGCGCGcGCUgggguGCGCcaAGCGGc -3' miRNA: 3'- aUGUCGUGCcCGAauu--UGCGa-UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 23147 | 0.66 | 0.966165 |
Target: 5'- gGCuGCgGCGGGCacGGGCGCUGaCGAu -3' miRNA: 3'- aUGuCG-UGCCCGaaUUUGCGAUcGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 82461 | 0.66 | 0.966165 |
Target: 5'- cACaAGCGCGucGGCgUGAGCGCcGGCGc -3' miRNA: 3'- aUG-UCGUGC--CCGaAUUUGCGaUCGCu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 120385 | 0.66 | 0.966165 |
Target: 5'- aUugAGC-CGGcGCUcGAGCGC-GGCGGu -3' miRNA: 3'- -AugUCGuGCC-CGAaUUUGCGaUCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 45431 | 0.66 | 0.966165 |
Target: 5'- uUGCuGGCGCGGcGCUUAucagUGCUaaGGCGGa -3' miRNA: 3'- -AUG-UCGUGCC-CGAAUuu--GCGA--UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 94795 | 0.66 | 0.966165 |
Target: 5'- cUGCGGCcCGGGCUcGGACGCa----- -3' miRNA: 3'- -AUGUCGuGCCCGAaUUUGCGaucgcu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 70235 | 0.66 | 0.964801 |
Target: 5'- gACAGCACGGGCgucaaaaaGUgGGCa- -3' miRNA: 3'- aUGUCGUGCCCGaauuug--CGaUCGcu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 101660 | 0.66 | 0.962685 |
Target: 5'- gUGCAGCGCGGGCaggUUGAugaaguuguCGCUcGCc- -3' miRNA: 3'- -AUGUCGUGCCCG---AAUUu--------GCGAuCGcu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 22416 | 0.66 | 0.962685 |
Target: 5'- aGCGGUACGGGC----GCGUcGGCa- -3' miRNA: 3'- aUGUCGUGCCCGaauuUGCGaUCGcu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 28705 | 0.66 | 0.962685 |
Target: 5'- aAUAGCGCucGCUgcAGCGCUgcaAGCGAu -3' miRNA: 3'- aUGUCGUGccCGAauUUGCGA---UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 86308 | 0.66 | 0.962685 |
Target: 5'- gUGCGGCGCGccgcGGCgccgucgAAGCGCUuGCGc -3' miRNA: 3'- -AUGUCGUGC----CCGaa-----UUUGCGAuCGCu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 106931 | 0.66 | 0.958965 |
Target: 5'- gGCGGCcuCGGGCaaauauggcAAACGCgucGGCGAa -3' miRNA: 3'- aUGUCGu-GCCCGaa-------UUUGCGa--UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 14379 | 0.66 | 0.958965 |
Target: 5'- aGCAGCccucaucgACGGGC--AGGCGUUGGgGGa -3' miRNA: 3'- aUGUCG--------UGCCCGaaUUUGCGAUCgCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 36822 | 0.66 | 0.958965 |
Target: 5'- aACGGCACGGGUuauuuuaguUUAGGCcacuugGGCGAc -3' miRNA: 3'- aUGUCGUGCCCG---------AAUUUGcga---UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 37828 | 0.67 | 0.950777 |
Target: 5'- aACAGCGCGcGGCgac-ACGCgUGGUGc -3' miRNA: 3'- aUGUCGUGC-CCGaauuUGCG-AUCGCu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 22880 | 0.67 | 0.941564 |
Target: 5'- --aGGCACGGGCc----CGCU-GCGAa -3' miRNA: 3'- augUCGUGCCCGaauuuGCGAuCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 61006 | 0.67 | 0.941564 |
Target: 5'- gGCGGCgGCGgcGGCgguGGCGCUuaGGCGAa -3' miRNA: 3'- aUGUCG-UGC--CCGaauUUGCGA--UCGCU- -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 91140 | 0.67 | 0.936565 |
Target: 5'- -uCGGCGCGcGGUcgcgUGcGCGCUGGCGc -3' miRNA: 3'- auGUCGUGC-CCGa---AUuUGCGAUCGCu -5' |
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21204 | 3' | -52.2 | NC_004778.3 | + | 98881 | 0.67 | 0.936565 |
Target: 5'- cGCGGCacGCGGGCUUu--CGa-AGCGAc -3' miRNA: 3'- aUGUCG--UGCCCGAAuuuGCgaUCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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