Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21219 | 5' | -53.3 | NC_004778.3 | + | 44083 | 0.66 | 0.964262 |
Target: 5'- gUCAUgGAcGCgGGCGuuuGCGGCGGCGAu -3' miRNA: 3'- aGGUGgCUuUGaCUGC---UGUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 81839 | 0.66 | 0.964262 |
Target: 5'- gCCGCC---AUUGGCGgaucgggaGCGGCGGCGGu -3' miRNA: 3'- aGGUGGcuuUGACUGC--------UGUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 119946 | 0.66 | 0.960743 |
Target: 5'- uUCCGCCGcacgugaaccuGugUGACGACGcGCAGgcaGAg -3' miRNA: 3'- -AGGUGGCu----------UugACUGCUGU-CGUCg--CU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 1266 | 0.66 | 0.956993 |
Target: 5'- gCUugUGGg---GACGACGGCGGCGu -3' miRNA: 3'- aGGugGCUuugaCUGCUGUCGUCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 46231 | 0.66 | 0.953005 |
Target: 5'- gCCGCUGAAAUguugcacaacaUGAacggcgcacaGACGGCAGCGu -3' miRNA: 3'- aGGUGGCUUUG-----------ACUg---------CUGUCGUCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 71263 | 0.66 | 0.953005 |
Target: 5'- -gCAuUCGGAcACggcGGCGGCGGCGGCGAc -3' miRNA: 3'- agGU-GGCUU-UGa--CUGCUGUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 14359 | 0.66 | 0.953005 |
Target: 5'- aUCGCUGAcgAAgUGGUGACAGCAGCc- -3' miRNA: 3'- aGGUGGCU--UUgACUGCUGUCGUCGcu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 103375 | 0.66 | 0.948775 |
Target: 5'- -gCGCCGugGCc-ACGACGGCGGUGGc -3' miRNA: 3'- agGUGGCuuUGacUGCUGUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 100844 | 0.66 | 0.948775 |
Target: 5'- gCCGCCGAug--GACGGCagaauuuugAGguGCGGa -3' miRNA: 3'- aGGUGGCUuugaCUGCUG---------UCguCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 309 | 0.66 | 0.948775 |
Target: 5'- cCCACCuGAGcgcguGCUGGCGcaagaauuugUAGCAGCGGu -3' miRNA: 3'- aGGUGG-CUU-----UGACUGCu---------GUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 119996 | 0.66 | 0.948775 |
Target: 5'- gCCGCCGA----GACGACGGCcGUGu -3' miRNA: 3'- aGGUGGCUuugaCUGCUGUCGuCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 100260 | 0.67 | 0.939576 |
Target: 5'- gCUGCCG-AGC-GACGACccguGGCAGCGu -3' miRNA: 3'- aGGUGGCuUUGaCUGCUG----UCGUCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 121749 | 0.67 | 0.939576 |
Target: 5'- --aACCGugcAACUGA--ACAGCGGCGAg -3' miRNA: 3'- aggUGGCu--UUGACUgcUGUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 75149 | 0.67 | 0.934603 |
Target: 5'- gCCGCCGggGCgGGCucacuGGCcGCAGCu- -3' miRNA: 3'- aGGUGGCuuUGaCUG-----CUGuCGUCGcu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 38634 | 0.67 | 0.929378 |
Target: 5'- uUCCAUUGugGCUG-CgGGCAGUAGCGu -3' miRNA: 3'- -AGGUGGCuuUGACuG-CUGUCGUCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 17096 | 0.67 | 0.929378 |
Target: 5'- gUCGgCGAGGCUG-CGACAGCA-UGAg -3' miRNA: 3'- aGGUgGCUUUGACuGCUGUCGUcGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 118108 | 0.67 | 0.9239 |
Target: 5'- gCCAUCGAGGaguUUGAcaaaauCGGCGGCGGCGc -3' miRNA: 3'- aGGUGGCUUU---GACU------GCUGUCGUCGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 36423 | 0.67 | 0.91817 |
Target: 5'- aUCCugCGuuggGGCUGcugcgcguucacGCGGcCGGCGGCGAc -3' miRNA: 3'- -AGGugGCu---UUGAC------------UGCU-GUCGUCGCU- -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 111251 | 0.68 | 0.912188 |
Target: 5'- cCCACCacGGAACUGGCuuuGAUAGCAauGCGc -3' miRNA: 3'- aGGUGG--CUUUGACUG---CUGUCGU--CGCu -5' |
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21219 | 5' | -53.3 | NC_004778.3 | + | 68055 | 0.68 | 0.912188 |
Target: 5'- uUCCGCUcAAACUGGCGcuCAGCGaCGAc -3' miRNA: 3'- -AGGUGGcUUUGACUGCu-GUCGUcGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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