Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21751 | 5' | -54.1 | NC_004813.1 | + | 35760 | 0.66 | 0.737517 |
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Target: 5'- gGACCGUgaauaUGCC--ACCGCACCCguuugugCGCc -3' miRNA: 3'- aUUGGCA-----GUGGuuUGGCGUGGGa------GUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 35308 | 0.66 | 0.748127 |
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Target: 5'- --uCCG-CACCGGACUGCAUCCg--- -3' miRNA: 3'- auuGGCaGUGGUUUGGCGUGGGagug -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 41805 | 0.66 | 0.768972 |
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Target: 5'- aGGCCGUCAU--GGCCGUcaGCUCUgACa -3' miRNA: 3'- aUUGGCAGUGguUUGGCG--UGGGAgUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 20751 | 0.66 | 0.768972 |
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Target: 5'- aGGCCGaacUCACCGGGagGCACCCggCAUc -3' miRNA: 3'- aUUGGC---AGUGGUUUggCGUGGGa-GUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 49087 | 0.66 | 0.779182 |
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Target: 5'- --cCCGUCACCAAACCugACCUgCAa -3' miRNA: 3'- auuGGCAGUGGUUUGGcgUGGGaGUg -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 15841 | 0.66 | 0.779182 |
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Target: 5'- -uGCUGUCAUUuuuGCCGCGCgugCCUUACc -3' miRNA: 3'- auUGGCAGUGGuu-UGGCGUG---GGAGUG- -5' |
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| 21751 | 5' | -54.1 | NC_004813.1 | + | 31388 | 0.66 | 0.779182 |
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Target: 5'- ----gGUUACCGcuugaGGCCGUGCCCUuCACa -3' miRNA: 3'- auuggCAGUGGU-----UUGGCGUGGGA-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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